| Field | Specification |
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| Mfr No | |
| Product Format | Frozen |
| Product Type | |
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Overview
Immortalized Rat Hippocampal Cells (H19-7) are derived from hippocampi that were dissected from E17 Holtzman rat embryos and subsequently immortalized via retroviral infection with tsA58 and U19tsa temperature sensitive mutants of the simian virus 40 large tumor antigen gene. These cells exhibit polygonal morphology prior to differentiation, after which they became elongated with thickened cell bodies and multiple extended processes.
Key elements and design rationale
- Model identity: Immortalized Rat Hippocampal Cells (H19-7) is supplied as an immortalized cell line derived from Rat hippocampus.
- Growth properties: Adherent, polygonal (becomes elongated post-differentiation)
- Growth conditions: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS(Regular*) + 1% L-glutamine (G275) + 0.2 mg/ml G418 (G271) + 1% Penicillin/Streptomycin Solution (G255), 33.0°C, 5% CO₂ *Do not heat-inactivate
- Engineering / immortalization: Immortalized via retroviral transduction of the tsA58 and U19tsa simian virus 40 large tumor antigen
- Product format: Frozen, BSL-2
This cell-based model is generally used in neurobiology, differentiation, and cell signaling studies.
Biological background
Differentiation of H19-7 cells depends upon the inactivation of the ts large tumor antigen and the environmental conditions. It was found that H19-7 cease DNA replication and cell division upon differentiation and are responsive to factors secreted by primary glia. H19-7 cells are useful tools in analyzing the development and regulation of hippocampal trophic interactions, an extremely interesting area given that the hippocampal formation expresses trophic signals that support the development and maintenance of synaptic inputs from the septal region, which in turn are invaluable for normal cognition and are particularly vulnerable in diseases such as Alzheimer's. Immunostaining used to detect for markers.
Research relevance and current trends
- Cultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.
- Researchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.
- Interpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.
Common research applications
- Neurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.
- Cell-based assays that compare experimental perturbations across defined media and substrate conditions.
- Phenotype tracking using morphology, marker expression, or reporter output where applicable.
Changes in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.
Notes for experimental interpretation
- Morphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.
- Matched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.
Culture and product details
- Growth Conditions: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS(Regular*) + 1% L-glutamine (G275) + 0.2 mg/ml G418 (G271) + 1% Penicillin/Streptomycin Solution (G255), 33.0°C, 5% CO₂ *Do not heat-inactivate
- Population Doubling Time (h): 22 - 31
- Immortalization Method: Immortalized via retroviral transduction of the tsA58 and U19tsa simian virus 40 large tumor antigen
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
How should I handle live cells once I receive them?
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
Why are these cells classified as biosafety level II?
What is your warranty or return policy?
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
How many times can cells divide?
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
