| Field | Specification |
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| Organism | |
| Applications | |
| Tissue | |
| Age | |
| Growth properties | |
| Biosafety level | |
| Product format | Frozen |
| Storage | |
| Shipping | |
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Overview
Immortalized Rat Neural Stem Cells (1464R) are spontaneously immortal population of neural stem cells derived from the brain stem. The cells retained morphology even after 50 passages.
Key elements and design rationale
- Model identity: Immortalized Rat Neural Stem Cells (1464R) is supplied as an immortalized cell line derived from Rat brain.
- Growth properties: Suspension
- Growth conditions: Cultures can be grown on poly(2-hydroxyethylmethacrylate) (PHEMA, #P3932; Sigma) coated cell culture vessels to prevent cell attachment. Neurobasal medium (Gibco) + 2 mM L-Glutamine (G275) + 2% B27 supplement (Thermo Fisher Scientific, Waltham, CA, USA) + 10 ng/mL of FGF2 (Z101455) + 10 ng/mL of EGF (Z100139) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. For information regarding cell differentiation, please refer to the "Differentiation Protocol" PDF under the Documents Tab.
- Engineering / immortalization: Spontaneous immortalization
- Product format: Frozen, BSL-2
This cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor/background information is available for contextual interpretation.
Biological background
In the presence of retinoic acid, the cells may differentiate to TuJ1-positive neurons and to GFAP-positive astrocytes or O4-positive oligodendrocytes. May be used for brain disease studies, and a source for neuronal and glial cells. Donor/background information provided for this product: 12 weeks old.
Research relevance and current trends
- Cultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.
- Researchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.
- Interpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.
Common research applications
- Neurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.
- Cell-based assays that compare experimental perturbations across defined media and substrate conditions.
- Phenotype tracking using morphology, marker expression, or reporter output where applicable.
Changes in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.
Notes for experimental interpretation
- Morphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.
- Matched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.
Culture and product details
- Growth Conditions: Cultures can be grown on poly(2-hydroxyethylmethacrylate) (PHEMA, #P3932; Sigma) coated cell culture vessels to prevent cell attachment. Neurobasal medium (Gibco) + 2 mM L-Glutamine (G275) + 2% B27 supplement (Thermo Fisher Scientific, Waltham, CA, USA) + 10 ng/mL of FGF2 (Z101455) + 10 ng/mL of EGF (Z100139) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. For information regarding cell differentiation, please refer to the "Differentiation Protocol" PDF under the Documents Tab.
- Immortalization Method: Spontaneous immortalization
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🧊 Thawing Protocol
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
🔬 Subculture Protocol
- Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10⁶ cells/ml.
- Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
How should I handle live cells once I receive them?
Please refer to our Cell Handling and Thawing Guidelines for detailed instructions on receiving, thawing, and culturing live cells:
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
Why are these cells classified as biosafety level II?
We follow the CDC-NIH recommendations that all mammalian sourced products should be handled at the Biological Safety Level 2 to minimize exposure of potentially infectious products. This information can be found in 'Biosafety in Microbiological and Biomedical Laboratories' (1999). Your institution's Safety Officer or Technical Services will be able to make the call as to whether BioSafety Level I is possible with these cells at your site, if desired.
What is your warranty or return policy?
Our warranty and return policy is outlined in abm’s Terms and Conditions, including details on product quality, limitations, and claims.
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
How many times can cells divide?
The number of times cells can divide depends on the cell type:
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Please refer to the Growth Conditions section of your specific product page. This section will indicate whether Applied Cell Extracellular Matrix (G422), PriCoat™ flasks, or both are recommended for optimal cell attachment and growth, as requirements may vary by cell type.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
Watabe, K., Akiyama, K., Kawakami, E., Ishii, T., Endo, K., Yanagisawa, H., ... & Tsukamoto, M. (2014). Adenoviral expression of TDP‐43 and FUS genes and shRNAs for protein degradation pathways in rodent motoneurons in vitro and in vivo. Neuropathology, 34(1), 83-98.
Ishii, T., Kawakami, E., Endo, K., Misawa, H., & Watabe, K. (2017). Myelinating cocultures of rodent stem cell line‐derived neurons and immortalized Schwann cells. Neuropathology, 37(5), 475-481.
Ishii, T., Kawakami, E., Endo, K., Misawa, H., & Watabe, K. (2017). Formation and spreading of TDP-43 aggregates in cultured neuronal and glial cells demonstrated by time-lapse imaging. PLoS One, 12(6), e0179375.
Watabe, K., Kato, Y., Sakuma, M., Murata, M., Niida‐Kawaguchi, M., Takemura, T., ... & Shibata, N. (2020). Praja1 RING‐finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP‐43 aggregate formation. Neuropathology, 40(6), 570-586.
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