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| Product format | Frozen |
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Overview
This novel, immortalized ecrine sweat gland myoepithelial cell line (iEM) serves as a model for research on sweat glands and sweating. These cells were developed from primary cells transduced with lentiviral vectors containing hTERT and SV40Tt.
Key elements and design rationale
- Model identity: Immortalized Sweat Gland Myoepithelial Cell Line (iEM) is supplied as an engineered cell line derived from Human sweat gland.
- Growth properties: Suspension, round spheroid
- Growth conditions: Ultra-Low Attachment Plates or upright culture flasks are required for cell growth; cells must remain in suspension in order to form spheroids. Mammocult Human Medium Kit (Stemcell Technologies, Cat. 05620) + 10 ng/ml EGF (Z100139) + 10 ng/ml bFGF (Z101456) + 4 µg/ml heparin + 0.5 µg/ml hydrocortisone 21-hemisuccinate + 2% (v/v) growth factor reduced matrigel (optional) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: thawed cells should be re-suspended in media supplemented with serum to neutralize DMSO. Cells take additional time to recover post-thaw and display cell death during the initial recovery phase. Grow cells in ultra-low attachemnt plates OR upright flask to allow spheroids to form. Subculture every 7 days and at least twice before experimental use. Seeding density: 2500 cells/well.
- Product format: Frozen, BSL-2
This cell-based model is generally used in sweat gland biology, phenotype comparison, and assay development studies. Donor/background information is available for contextual interpretation.
Biological background
Cells display spheroid formation with increased population doubling compard to non-transduced cells. iEM's express myoepithelial cell-specific proteins (a-SMA, B1 or K14) and can differentiate into luminal cells. Immortalized Sweat Gland Myoepithelial Cells provide a useful in vitro model for recapitulating eccrine sweat gland functions and for screening substances that can modulate sweating by acting on sweat glands. These cells are applicable for studies in hyperhidrosis, adiaphoresis, and sweat gland diseases. Please note: cells are exected to grow to 65 population doublings (PDs), however, the exact PD limit can vary by lot. Donor/background information provided for this product: Female, 53, Eyelid.
Research relevance and current trends
- Engineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.
- Reporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.
- Expression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.
Common research applications
- Routine expansion and maintenance of a defined cell model for downstream in vitro experiments.
- Phenotype, signaling, or marker-expression studies performed under standardized culture conditions.
- Cell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.
Changes in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.
Notes for experimental interpretation
- Morphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.
- Matched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.
Culture and product details
- Growth Conditions: Ultra-Low Attachment Plates or upright culture flasks are required for cell growth; cells must remain in suspension in order to form spheroids. Mammocult Human Medium Kit (Stemcell Technologies, Cat. 05620) + 10 ng/ml EGF (Z100139) + 10 ng/ml bFGF (Z101456) + 4 µg/ml heparin + 0.5 µg/ml hydrocortisone 21-hemisuccinate + 2% (v/v) growth factor reduced matrigel (optional) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: thawed cells should be re-suspended in media supplemented with serum to neutralize DMSO. Cells take additional time to recover post-thaw and display cell death during the initial recovery phase. Grow cells in ultra-low attachemnt plates OR upright flask to allow spheroids to form. Subculture every 7 days and at least twice before experimental use. Seeding density: 2500 cells/well.
- Population Doubling Time (h): 24 - 46
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media supplemented with 10% FBS. Centrifuge cells at 300xg for 5 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10⁶ cells/ml.
- Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
How should I handle live cells once I receive them?
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
Why are these cells classified as biosafety level II?
What is your warranty or return policy?
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
How many times can cells divide?
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
