| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cell proliferation inducing protein 52 antibody; Cell proliferation-inducing gene 4/52 protein antibody; Heart muscle protein antibody; HMP antibody; IMMT antibody; IMMT_HUMAN antibody; Inner membrane protein mitochondrial antibody; Inner mitochrondial membrane antibody; MICOS complex subunit MIC60 antibody; MINOS2 antibody; Mitochondrial inner membrane organizing system 2 antibody; Mitochondrial inner membrane protein antibody; Mitofilin antibody; Motor protein antibody; p87 antibody; p87/89 antibody; p89 antibody; pig4 antibody; PIG52 antibody; Proliferation-inducing gene 4 antibody |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human IMMT protein |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a recombinant monoclonal antibody expressed in HEK293F cells. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a monoclonal anti-IMMT antibody raised in Human, with confirmed utility in ELISA, IHC, FC. It is designed to detect IMMT protein in Human and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Recombinant Human IMMT protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Human): Requires anti-human-IgG secondary reagents for indirect detection.
- Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
- Isotype (IgG1): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Affinity chromatography): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
IMMT (also referred to as Cell proliferation inducing protein 52 antibody, Cell proliferation-inducing gene 4/52 protein antibody, Heart muscle protein antibody, HMP antibody, IMMT antibody, IMMT_HUMAN antibody, Inner membrane protein mitochondrial antibody, Inner mitochrondial membrane antibody, MICOS complex subunit MIC60 antibody, MINOS2 antibody, Mitochondrial inner membrane organizing system 2 antibody, Mitochondrial inner membrane protein antibody, Mitofilin antibody, Motor protein antibody, p87 antibody, p87/89 antibody, p89 antibody, pig4 antibody, PIG52 antibody, Proliferation-inducing gene 4 antibody) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of IMMT biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- IHC: Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.
- FC: Applicable for FC-based detection; refer to product documentation for optimized conditions.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG1 from Human) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a recombinant monoclonal antibody expressed in HEK293F cells.
IMMT is a protein target in Human biology. This monoclonal antibody raised in Human is designed to detect IMMT in ELISA, IHC, FC applications, with IgG1 isotype.
Reported reactive against Human. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, IHC, FC. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG1 antibody requires a compatible secondary. Select anti-human IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG1.
(1) Isotype control — IgG1 from Human at matching concentration; (2) Positive control — known IMMT-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
