| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | INSL 3; INSL-3; INSL3_HUMAN; Insulin like 3; Insulin-like 3 A chain; Ley IL; Ley-I-L; Leydig insulin like peptide; Leydig insulin-like peptide; Relaxin like factor; Relaxin-like factor; RLF; RLNL |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 ofhuman INSL3 (NP_005534.2). |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
Research Use OnlyInsulin-like factor 3 (INSL3), also designated Leydig insulin-like peptide (Ley IL) and Relaxin-like factor (RLF), is a peptide hormone in the Relaxin family which is secreted from the testicular Leydig cells and ovarian theca interna cells. INSL3 is involved in gonadal and other physiological processes. Structurally similar to Relaxin and insulin, INSL3 differs from the two in that it signals through a G protein-coupled receptor, LGR8.
Validated Applications
| Immunohistochemistry (IHC) | 50 - 1:100 |
|---|---|
| Immunofluorescence (IF/ICC) | 50 - 1:100 |
| ELISA (ELISA) | 5000 - 1:10000 |
Key Specifications
| Host Species | Rabbit |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 15 kDa |
| Catalog No. | BS7819 |
| UniProt | P51460 |
| Alternative Names | INSL 3; INSL-3; INSL3_HUMAN; Insulin like 3; Insulin-like 3 A chain; Ley IL; Ley-I-L; Leydig insulin like peptide; Leydig insulin-like peptide; Relaxin like factor; Relaxin-like factor; RLF; RLNL |
Immunogen / Antigen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 ofhuman INSL3 (NP_005534.2).
Reactivity Notes
INSL3 polyclonal antibody detects endogenous levels of INSL3 protein.
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Immunohistochemistry, Immunofluorescence, ELISA. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
IHC-P 1:50 - 1:100 IF/ICC 1:50 - 1:100 ELISA 1:5000 - 1:10000
This antibody has been confirmed reactive against: Human, Mouse, Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 ofhuman INSL3 (NP_005534.2).
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.