| Field | Specification |
|---|---|
| Target | |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C23H22N6O3 |
| SMILES | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
IRAK4-IN-27 is a potent, selective inhibitor of IRAK4 with an IC50 of 8.7 nM. It suppresses cell growth and promotes apoptosis in the MYD88 L265P diffuse large B-cell lymphoma (DLBCL) cell line, supporting its use in DLBCL research[1]. It has the molecular formula C23H22N6O3 and a molecular weight of 430.46 g/mol.
Physical & Chemical Properties
| CAS Number | 2626988-86-9 |
|---|---|
| Molecular Formula | C23H22N6O3 |
| Molecular Weight | 430.46 g/mol |
| SMILES | O=C(C1=COC(C2=CC(N)=NC=C2)=N1)NC3=C(C4=CN(C)N=C4)C=C(OC(C)(C)C5)C5=C3 |
| Target | IRAK4 |
| Signaling Pathway | Immunology/Inflammation; Apoptosis |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target
|
IRAK4 8.7 nM (IC50) |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with your institution's chemical hygiene plan.
In Vitro
Antiproliferative activity against the MYD88 L265P DLBCL cell line (OCI-LY10, IC50 = 0.248 μM) and the MYD88 WT cell lines (U2932, IC50 = 1.251 μM, and GM00637, IC50 = 1.520 μM) is shown by IRAK4-IN-27 (72 h) [1]. Apoptosis is induced in OCI-LY10 cells by IRAK4-IN-27 (0.5 and 1 μM, 24 and 48 h)[1]. In OCI-LY10 cells, IRAK4-IN-27 (0.03-3 μM) suppresses the phosphorylation of IRAK4 together with downstream signaling[1]. Combined with Ibrutinib, IRAK4-IN-27 (0.1 and 0.2 μM, 48 h) inhibits cell growth but induces apoptosis in OCI-LY10 cells[1].
Apoptosis Analysis[1]
| Cell Line | OCI-LY10 cells |
|---|---|
| Concentration | 0, 0.1, 0.2, 0.5, 1 μM |
| Incubation Time | 24 and 48 h |
| Result | Triggered significant apoptosis after 24 h incubation, with apoptosis rates of 48.3% and 66.8% at the concentration of 0.5 μM and 1 μM, respectively. Induced apoptosis of 67.2% and 92.3% after 48 h incubation at the concentration of 0.5 μM and 1 μM, respectively. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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