| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
IRF1 (interferon regulatory factor 1) is a transcription factor that coordinates innate and adaptive immune responses. Activated downstream of pattern-recognition receptors such as Toll-like, RIG-I-like, NOD-like, and C-type lectin receptors, IRF family members translocate to the nucleus and bind interferon-stimulated response element-like sequences. IRF1 induces interferons and immune effector genes, supports antiviral and antibacterial defense, and contributes to immune surveillance against intracellular threats. It also shapes the development and function of NK cells, macrophages, and Th1/CD8+ T cells. IRF response elements integrate signals from AP-1, IRF, and NF-κB motifs, allowing IRF1 reporters to capture cytokine- and pathogen-driven transcriptional activation.
Product Description & Applications
The IRF1 Reporter Lentivirus is a transcription-factor reporter system for detecting IRF1-mediated transcriptional activity. It contains tandem repeats of IRF response elements derived from the human IFNβ enhancer, comprising AP-1, IRF, and NF-κB motifs, coupled to a minimal TATA-box promoter and an upstream enhancer that maximizes signal-to-noise to drive a fluorescent or luminescent reporter (including BFP2, d2GFP, EGFP, GFP, mCherry, RFP, firefly, Gaussia, or Renilla luciferase). A constitutively expressed selection marker (blasticidin, hygromycin, puromycin, or zeocin) supports stable polyclonal cell line generation. Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved material, with readout by microscopy, flow cytometry, or luminometry. It is used to study IRF1 activation in cell-signaling and immunology research. Supplied as purified lentiviral particles.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the IRF1 Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
