| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human IRF2BP1 recombinant protein (Position: A71-D439) was used as the immunogen for the IRF2BP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IRF2BP1 Antibody / Interferon regulatory factor 2-binding protein 1 is a anti-IRF2BP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: IRF2BP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IP, IF, IHC, ICC, WB
Biological background
IRF2BP1 plays an essential role in maintaining immune homeostasis and regulating the magnitude of antiviral and inflammatory responses. By modulating IRF2 and IRF1 target genes, it fine-tunes the balance between transcriptional activation and repression in interferon signaling pathways. IRF2BP1 also participates in the negative regulation of p53 and NF-?B target genes, thereby linking interferon signaling to cell survival and immune control. Its activity is regulated through post-translational modifications such as phosphorylation and SUMOylation, which alter subcellular localization and cofactor binding.
The IRF2BP1 antibody is widely used in immunology, virology, and transcriptional regulation studies to detect IRF2BP1 expression and nuclear localization. Western blot analysis typically identifies a 58 kilodalton band, and immunofluorescence reveals nuclear and perinuclear distribution patterns consistent with its function as a transcriptional repressor. This antibody enables researchers to examine the interplay between interferon signaling and chromatin regulation, as well as transcriptional repression during immune activation.
Dysregulation of IRF2BP1 expression has been associated with aberrant immune responses, chronic inflammation, and oncogenesis. It acts as a checkpoint that restrains excessive interferon responses, preventing autoimmunity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
