ITCH Antibody, Biotin conjugated

SKU:BHA10561889
Overview
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ITCH-specific polyclonal antibody raised in Rabbit. Biotin-conjugated. Validated for ELISA in Human samples. Affinity-purified.
Conjugate Biotin
Immunogen Recombinant
Applications 1 validated
Target Aliases 23 aliases
Options selector
Catalog no. Size
CSB-PA839346HD01HU-50UL 50 uL
CSB-PA839346HD01HU-100UL 100 uL
Available Options
Size
Field Specification
Mfr No CSB-PA839346HD01HU
Alternative Names ADMFD; AIF4; AIP4; Atrophin 1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)); dJ468O1.1 atrophin 1 interacting protein 4 AIP4; E3 ubiquitin protein ligase Itchy homolog; E3 ubiquitin-protein ligase Itchy homolog; EC 6.3.2; Itch; ITCH_HUMAN; Itchy E3 ubiquitin protein ligase; Itchy E3 ubiquitin protein ligase homolog; Itchy E3 ubiquitin protein ligase homolog mouse; Itchy E3 ubiquitin protein ligase, mouse, homolog of; Itchy homolog E3 ubiquitin protein ligase; Itchy mouse homolog E3 ubiquitin protein ligase; NAPP1; NFE2 associated polypeptide 1; NFE2-associated polypeptide 1; Ubiquitin protein ligase ITCH
Antigen
  • Recombinant Human E3 ubiquitin-protein ligase Itchy homolog protein (29-303AA)
Clonality
  • Polyclonal
Conjugate
  • Biotin
Form Liquid
Host Rabbit
Immunogen Recombinant Human E3 ubiquitin-protein ligase Itchy homolog protein (29-303AA)
Isotype
  • IgG
Product Type
  • Primary Antibody
Reactivity
  • Human
Source This product is a polyclonal antibody purified from rabbit antiserum.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target ITCH
UniProt # Q96J02

Product Overview

Primary Antibody

This antibody targets ITCH and was raised against a recombinant protein immunogen.

Validated Applications

Application Recommended Dilution
ELISA Refer to datasheet
✔ Validated by CUSABIO Technology

Antibody Specifications

Target ITCH
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Biotin
Reactivity Human
Purification Protein G Purification
Antigen / Immunogen Recombinant Human E3 ubiquitin-protein ligase Itchy homolog protein (29-303AA)
Production This product is a polyclonal antibody purified from rabbit antiserum.
Form Liquid
Storage Buffer 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names

ADMFD; AIF4; AIP4; Atrophin 1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)); dJ468O1.1 atrophin 1 interacting protein 4 AIP4; E3 ubiquitin protein ligase Itchy homolog; E3 ubiquitin-protein ligase Itchy homolog; EC 6.3.2; Itch; ITCH_HUMAN; Itchy E3 ubiquitin protein ligase; Itchy E3 ubiquitin protein ligase homolog; Itchy E3 ubiquitin protein ligase homolog mouse; Itchy E3 ubiquitin protein ligase, mouse, homolog of; Itchy homolog E3 ubiquitin protein ligase; Itchy mouse homolog E3 ubiquitin protein ligase; NAPP1; NFE2 associated polypeptide 1; NFE2-associated polypeptide 1; Ubiquitin protein ligase ITCH

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle with standard laboratory precautions. Dispose per local regulations.
TargetITCH
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ConjugateBiotin
ReactivityHuman
PurificationProtein G Purification
Antigen/ImmunogenRecombinant Human E3 ubiquitin-protein ligase Itchy homolog protein (29-303AA)
ProductionThis product is a polyclonal antibody purified from rabbit antiserum.
FormLiquid
Storage Buffer0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Dilutions are starting recommendations. Titration is advised for each system.

ApplicationRecommended Dilution
ELISARefer to datasheet

Immunogen: Recombinant Human E3 ubiquitin-protein ligase Itchy homolog protein (29-303AA)

Antigen Species: Human

Alternative Names

ADMFD; AIF4; AIP4; Atrophin 1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)); dJ468O1.1 atrophin 1 interacting protein 4 AIP4; E3 ubiquitin protein ligase Itchy homolog; E3 ubiquitin-protein ligase Itchy homolog; EC 6.3.2; Itch; ITCH_HUMAN; Itchy E3 ubiquitin protein ligase; Itchy E3 ubiquitin protein ligase homolog; Itchy E3 ubiquitin protein ligase homolog mouse; Itchy E3 ubiquitin protein ligase, mouse, homolog of; Itchy homolog E3 ubiquitin protein ligase; Itchy mouse homolog E3 ubiquitin protein ligase; NAPP1; NFE2 associated polypeptide 1; NFE2-associated polypeptide 1; Ubiquitin protein ligase ITCH

What species does this ITCH antibody react with?
This antibody has been validated for reactivity against Human. Cross-reactivity is based on the immunogen sequence and may vary by sample type.
What applications has this antibody been validated for?
Validated applications include: ELISA. Each application was confirmed with appropriate controls. Contact technical support for applications not listed.
What is the recommended dilution?
Assay dependent. Optimal concentrations depend on sample type, detection system, and antigen abundance. Titration with positive and negative controls is recommended.
How was this antibody produced?
This polyclonal IgG antibody was raised in Rabbit and purified by affinity chromatography using Protein G. It provides high specificity and minimal cross-reactivity in standard immunoassay applications.
What are the storage requirements?
Store at −20°C for long-term. Avoid repeated freeze-thaw cycles. Short-term working aliquots may be stored at 4°C. This product is supplied in a stabilized buffer containing BSA and sodium azide.
Is this antibody directly conjugated and does it require secondary detection?
Yes, this antibody is Biotin-conjugated and can be used directly without a secondary antibody. It is ready to use in assays requiring Biotin detection, such as flow cytometry or direct ELISA applications. Ensure your detection instrument is calibrated for Biotin signal.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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