Jacalin Plates

Jacalin Plates, available in various formats, are designed for their specific affinity to molecules containing non-reducing α-D-galactosyl groups. These plates are ideal for binding carbohydrate-binding proteins and glycoproteins, particularly those found in IgA1 and cell membranes. Jacalin, a lectin derived from the seeds of Artocarpus integrifolia (jackfruit), is widely used for isolating glyco-conjugates and glycoproteins with distinct carbohydrate structures.

The Jacalin Coated 96 Well Plates are ideal for a variety of applications, including the specific binding of human IgA1 with steric orientation, the purification of human immunoglobulins (particularly IgA1), and the separation of antigen-antibody immunocomplexes. Additionally, they are used for isolating IgA1 from contaminants and stimulating T-cells. These features make the plates a versatile tool for both immunological and biochemical assays.

Key Features

• Enables specific binding of human IgA1 with precise steric orientation, ensuring accuracy in assays.
• Facilitates efficient purification of human immunoglobulins, particularly IgA1, improving yield and purity.
• Allows for effective separation of antigen-antibody immunocomplexes, enhancing assay sensitivity.
• Provides reliable isolation of IgA1 from contaminants, ensuring sample integrity.
• Supports T-cell stimulation, making it suitable for immunological research and applications.
• Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
• Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
• Wells design: flat bottom.
• Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
• Colors: Available in clear, white, and black. Customizable upper rim colors upon request. Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays. Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
  • Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
  • Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
• Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
• Alphanumeric Coding: For easy and accurate well identification during experiments.
• Recommended working volume: of 50 to 100 μl
• Packaging: Each coated plate is packed in a single barrier bag with desiccant
• Unit: Contains 5 plates
• Minimum order: 10 plates.
• Ready to use

Key Benefits

• Plates Design: The mould design provides optical quality, important to reduce the background signal. The rim protects the external face of the bottom from scratches
  • The mould design provides optical quality, important to reduce the background signal.
  • The rim protects the external face of the bottom from scratches
• Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
• Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
• Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
• Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Applications

• Human IgA1 specific binding, sterically oriented
• Purification of human immunoglobulins (especially IgA1)
• Separation of immunocomplexes antigen-antibody
• Separation of IgA1 from contaminants
• Stimulation of T-cells
• ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
• Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
• Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
• Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.