| Field | Specification |
|---|---|
| Target | |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C27H31N5O4S |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
JNK-IN-14 is a potent, selective JNK inhibitor with IC50 values of 1.81 nM, 12.7 nM, and 10.5 nM against JNK1, JNK2, and JNK3, respectively. It induces early-stage apoptosis and G2/M cell cycle arrest and can be used in cancer research[1]. It is supplied as a white to off-white solid (C27H31N5O4S, MW 521.63) at 99.92% purity.
Physical & Chemical Properties
| CAS Number | 2991432-56-3 |
|---|---|
| Molecular Formula | C27H31N5O4S |
| Molecular Weight | 521.63 g/mol |
| Purity | 99.92% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | CC(C)(N1C=C(C(C2=CC(O)=CC=C2)=N1)C3=CC=NC(NCCCNS(=O)(C4=CC=C(C=C4)O)=O)=C3)C |
| Target | JNK1, JNK2, JNK3, CYP2D6, CYP3A4 |
| Signaling Pathway | MAPK/ERK Pathway; Apoptosis |
| Solubility | In Vitro: DMSO: ≥ 100 mg/mL (191.71 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) * "≥" means soluble, but saturation unknown. |
| Storage | -20°C, stored under nitrogen. In solvent: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target
|
JNK1 1.82 nM (IC50) |
JNK2 10.5 nM (IC50) |
JNK3 10.5 nM (IC50) |
CYP2D6 26.7 nM (IC50) |
CYP3A4 383.0 nM (IC50) |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | ≥ 100 mg/mL (191.71 mM) | use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); stored under nitrogen; avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
JNK-IN-14 (2.5 μM-5 μM, 24 h) causes slight early-stage apoptosis in K562 cells in a concentration-dependent manner[1]. In K562 cells, JNK-IN-14 (1.25-5 μM, 24 h) induces arrest of the cell cycle at G2/M[1]. The GI50 of JNK-IN-14 against NCI K562 cells is 1.28 μM[1]. In K562 leukemia cells, JNK-IN-14 (2.5 μM-5 μM, 24 h) inhibits production of beclin-1[1].
Apoptosis Analysis[1]
| Cell Line | K562 Human Leukemia Cell |
|---|---|
| Concentration | 2.5 μM, 5 μM |
| Incubation Time | 24 h |
| Result | Resulted in early-stage apoptosis with 1.94%, and 2.14% for 2.5 and 5 μM, respectively. |
Cell Cycle Analysis[1]
| Cell Line | K562 human leukemia cell |
|---|---|
| Concentration | 1.25 μM, 2.56 μM, 5 μM |
| Incubation Time | 24 h |
| Result | Showed viable cells arrest at the G2/M phase with values of 45.33% at 1.25 μM, 2.5 μM at 62.99%, and 5 μM at 98.97%. |
Western Blot Analysis[1]
| Cell Line | K562 Cells |
|---|---|
| Concentration | 2.5 μM, 5 μM |
| Incubation Time | 24 h |
| Result | Inhibited beclin-1 production at higher concentrations in K562 leukemia cells. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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