K252b

Product details

• Chemical name: (9R,10S,12S)-2,3,9,10,11,12-Hexahydro-10-hydroxy-9-methyl-1-oxo-9,12-epoxy-1H-diindolo[1,2,3-fg:3',2',1'-kl]pyrrolo[3,4-i][1,6]benzodiazocine-10-carboxylic acid.
• CAS number: 99570-78-2
• Molecular formula: C26H19N3O5.
• Purity: >98%
• Concentration: 50-500 nM.
• Form: Lyophilized Powder
• Solubility: DMSO or DMF. Centrifuge all product preparations before use (10000 x g 5 min).
• Reconstitution: DMSO or DMF. Centrifuge all product preparations before use (10000 x g 5 min).
• Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
• Target: Protein Kinase A, Protein Kinase C, and Protein Kinase G
• Source: Natural
• Activity: K252b is a less potent derivative of K252a and inhibits a variety of kinases including PKC (IC50 = 20 nM), PKA (IC50 = 90 nM) and PKG (IC50 = 100 nM)1. It also inhibits MLCK (IC50 = 147 nM) and CaMK (IC50 = 12 nM).

Scientific background

K252b is an alkaloid isolated from Nocardiopsis sp. soil fungi. It is a less potent derivative of K252a,3-5 cell-permeable protein kinase inhibitor.This compound potentiates neurotrophin-3 activity in certain neurons by an unknown mechanism. In other neurons it serves as a control to K252a since it accumulates in the plasma membrane of the neuron.1

Lead time: 1-2 Business Days

Country of origin: Israel/IL

Applications key

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Bioassay tested: Yes

• SDS (PDF)

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