| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human K27-linkage Specific Ubiquitin was used as the immunogen for the K27-linkage Specific Ubiquitin antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
K27-linkage Specific Ubiquitin Antibody / UBB / UBC is a anti-K27-LINKAGE Rabbit antibody Recombinant Rabbit Monoclonal clone 31U80 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: K27-LINKAGE
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31U80, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Unlike the well characterized K48 and K63 chains, lysine 27 linked ubiquitin chains are less abundant but play specialized roles. Research using K27-linkage specific ubiquitin antibody has shown that these chains regulate mitochondrial homeostasis, DNA damage repair, and innate immune signaling. By targeting proteins for specific fates, K27 chains contribute to non-proteolytic ubiquitin functions, highlighting the complexity of the ubiquitin code.
In innate immunity, lysine 27 chains are involved in regulating antiviral signaling pathways such as RIG-I and MAVS. Studies with K27-linkage specific ubiquitin antibody have revealed that these chains promote activation of signaling complexes leading to interferon production. In DNA repair, K27 ubiquitin regulates assembly of repair factors at sites of damage. Dysregulation of K27 chains is linked to impaired genome stability and susceptibility to cancer.
Because ubiquitin modifications are dynamic and context dependent, linkage-specific antibodies are essential for dissecting the ubiquitin code. K27-linkage specific ubiquitin antibody allows researchers to distinguish lysine 27 linkages from other ubiquitin chains, enabling studies of how different linkages integrate into signaling networks. This specificity is crucial for understanding how ubiquitination encodes information beyond protein degradation.
K27-linkage specific ubiquitin antibody is applied in western blotting, immunoprecipitation, and immunofluorescence. Western blotting detects K27-linked chains in cellular extracts, immunoprecipitation isolates proteins modified with this linkage, and immunofluorescence reveals dynamic localization after stress or immune stimulation. These methods highlight its value in ubiquitin signaling research.
By supplying validated K27-linkage specific ubiquitin antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
