| Field | Specification |
|---|---|
| Species |
The K7M2 wt cell line is derived from a murine osteosarcoma and is frequently utilized in cancer research, particularly for studies investigating the pathogenesis and therapeutic response of osteosarcoma. This cell line is characterized by its high metastatic potential, making it an invaluable model for studying the mechanisms underlying cancer metastasis and for testing anti-metastatic agents. K7M2 wt cells display a typical epithelial morphology and exhibit robust growth in vitro, which facilitates various experimental applications including gene expression studies, drug screening, and genetic manipulation.
Researchers leverage the K7M2 wt cell line to explore the molecular and cellular processes involved in osteosarcoma progression. Studies often focus on signaling pathways, such as the Wnt/β-catenin and PI3K/AKT pathways, which are crucial in tumor growth and metastasis. The genetic profile of K7M2 wt cells includes alterations common in osteosarcoma, providing insights into the genetic drivers of this malignancy. Furthermore, this cell line is instrumental in preclinical testing of new therapeutic approaches, including targeted therapies and immunotherapies, offering a platform for translating research findings into potential clinical applications.
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- Receptors expressed: Complement(C3), expressed, Fc receptor, IgG, high affinity I(Fcgr1), expressed
- Tumorigenic: Yes
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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