KDM2B Antibody

Overview

This polyclonal anti-KDM2B antibody raised in Rabbit, tested in ELISA, IF. Designed to detect KDM2B in Human for epigenetics and nuclear signaling research.

Key elements and design rationale

• Immunogen: Recombinant Human Lysine-specific demethylase 2B protein (1274-1336AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.

Biological background

KDM2B (also: [Histone-H3]-lysine-36 demethylase 1B, CXXC-type zinc finger protein 2, CXXC2, F box and leucine rich repeat protein 10, F box protein FBL10, F-box and leucine-rich repeat protein 10, F-box protein FBL10, F-box/LRR-repeat protein 10, Fbl10, FBXL10, JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein, JHDM1B, JmjC domain-containing histone demethylation protein 1B, Jumonji C domain containing histone demethylase 1B, Jumonji domain-containing EMSY-interactor methyltransferase motif protein, kdm2b, KDM2B_HUMAN, Lysine (K) specific demethylase 2B, Lysine-specific demethylase 2B, PCCX2, Protein containing CXXC domain 2, Protein JEMMA, Protein-containing CXXC domain 2) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current KDM2B annotation relevant to epigenetics and nuclear signaling.

Common research applications

• ELISA: Quantify soluble target; ensure samples within the linear detection range.
• IF: Subcellular localization in fixed cells/sections; optimize fixation.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Human; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (12781408)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.