| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Klenow (3'→5'exo-) DNA polymerase is a proteolytic product of E. coli DNA polymerase I, retaining the DNA polymerase activity of DNA polymerase I but losing the 5'→3' exonuclease activity. This enzyme has further removed the 3'→5' exonuclease activity through mutation. It can be used for DNA labeling with random priming and DNA sequencing by the dideoxy method, among other applications.
Features
No nickase or exonuclease residue
Low residual host DNA
High amplification efficiency
Applications
Random primer labeling
Sanger dideoxy DNA sequencing
Synthesis of the second strand of cDNA
Synthesis of the second strand in mutagenesis reactions
Specifications
|
Source |
E. coli |
|
Molecular Weight |
68.1 KDa |
|
Concentration |
5 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme that incorporates 10 nmol of dNTP into acid-insoluble material within 30 minutes at 37°C |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
14460ES70 |
14460ES80 |
14460ES90 |
||
|
14460-A |
Klenow Fragment(3'→5' exo-) (5 U/μL) |
40 μL |
200 μL |
1 mL |
||
|
14460-B |
10×Klenow Fragment (exo-) Buffer |
250 μL |
1.25 mL |
5×1.25 mL |
||
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 2 years.
Figures
Figure1. Residual E.coli genomic DNA detection
Note:The residual E. coli genomic DNA in three batches of Klenow Fragment (3'→5' exo-) (Cat#14460) is below 1 copy/50 U.
Figure2. Product Stability Testing
Note: Klenow Fragment (3'→5' exo-) from Yeasen and Supplier A was stored at 37°C for 7 and 14 days to assess residual enzyme activity. The results indicate that the residual activity of Yeasen 's Klenow Fragment (3'→5' exo-) (Cat#14460) after 14 days at 37°C is notably higher than that of the Supplier A.
Figure3. NGS library yield
Note: Using the same amount of Klenow Fragment (3'→5' exo-) (Cat#14460) from Yeasen and Supplier A for NGS library construction, the results show that the library yield with Yeasen 's Klenow Fragment (3'→5' exo-) (Cat#14460) at both 5 U and 10 U input is higher than that of Supplier A.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
Random primer labeling Sanger dideoxy DNA sequencing Synthesis of the second strand of cDNA Synthesis of the second strand in mutagenesis reactions. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of deoxyribonucleotide into acid-insoluble material in 30 min under standard assay conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
This enzyme is produced recombinantly in E. coli under stringent quality controls and validated on multiple substrate types. Key performance parameters include specific activity, substrate specificity, cofactor requirements, optimal pH/temperature range, and compatibility with downstream assays. Refer to the product datasheet for validated lot-specific activity data and recommended reaction conditions.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
