| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C30H28N3O6+ |
| SMILES | |
| Form | Liquid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
KMG-301AM is the acetoxymethyl ester of KMG-301; it accumulates in mitochondria and is then hydrolyzed there to release KMG-301, a fluorescent probe selective for Mg2+ that functions within mitochondria of intact cells. Because the mitochondrial membrane is impermeable to KMG-301, the probe is not released upon depolarization of the mitochondrial membrane potential, and it can report changes in mitochondrial Mg2+ concentration and reveal Mg2+ transport across the mitochondrial membrane during the early phase of a cellular model[1]. It is supplied as a brown to red liquid (C30H28N3O6+, MW 526.56).
Physical & Chemical Properties
| CAS Number | 1372642-77-7 |
|---|---|
| Molecular Formula | C30H28N3O6+ |
| Molecular Weight | 526.56 g/mol |
| Appearance | Liquid |
| Color | Brown to red |
| SMILES | O=C1C(C(OCOC(C)=O)=O)=CC(C(C2=CC=C(N(C)C)C=C2O3)=C4C3=C/C(C=C4)=[N+](C)/C)=C5C=CC=CN51 |
| Storage | 4°C, protect from light. In solvent: -80°C, 6 months; -20°C, 1 month (protect from light). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
Guidelines: the protocol below is a recommended guideline only and should be modified to specific needs[1]. 1. For optical imaging, apply 20 μM KMG-301AM in Hanks' balanced salt solutions (HBSS) to the cells for 10 min on ice, which avoids hydrolysis of the acetoxymethyl ester by esterase in the cytosol. HBSS composition (mM): NaCl 137, KCl 5.4, CaCl2 1.3, MgCl2 0.5, MgSO4 0.4, Na2HPO4 0.3, KH2PO4 0.4, NaHCO3 4.2, D-glucose 5.6, HEPES 5; adjust pH to 7.4 with NaOH. 2. Wash the cells twice with HBSS and incubate for 15 min at 37°C to allow complete hydrolysis of the acetoxymethyl ester form in mitochondria. 3. For simultaneous use of KMG-104AM and KMG-301AM, incubate the cells with 5 μM KMG-104AM in HBSS for 30 min at 37°C, then stain with KMG-301AM. 4. Incubate isolated mitochondria for 20 min at 37°C with 20 μM KMG-301AM in Mitochondria Imaging Buffer (MIB). Wash the mitochondria twice with MIB and incubate for a further 15 min at 37°C. MIB composition (mM): KCl 125, K2HPO4 2, MgCl2 1, HEPES 5, EDTA 0.02; adjust pH to 7.2 with KOH. Alternatively, use Mg2+-free MIB (without MgCl2). 5. Perform fluorescence imaging experiments with a confocal laser scanning microscope system. Excite KMG-301 at 559 nm from a laser diode and observe the signal at 600-700 nm. For simultaneous measurement, excite KMG-104 at 488 nm from an Argon laser and KMG-301 at 559 nm. Separate the fluorescence with a 560 nm dichroic mirror and observe at 500-545 nm and 600-700 nm, respectively.
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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