| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A portion of amino acids 195-220 from the human protein was used as the immunogen for the KMT5C antibody. |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
KMT5C Antibody / SUV420H2 is an antibody targeting SUV420H2, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: SUV420H2.
- Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Purified.
- Species reactivity: Human, Mouse.
- Listed applications: WB, FACS (refer to on-page specifications for application-specific guidance).
Biological background
KMT5C, also called Suppressor of variegation 4-20 homolog 2, Su(var)4-20 homolog 2 and Suv4-20h2, belongs to a family of proteins called lysine methyltransferases, which are enzymes that play a key role in the modification of histone proteins. Histones are proteins that help package and organize DNA in the nucleus of a cell, and modifications to these proteins can have profound effects on gene expression. Studies have shown that KMT5C specifically targets histone H4 lysine 20 (H4K20) for methylation, which has been linked to the regulation of gene transcription and DNA replication. Dysregulation of this process has been implicated in various diseases, including cancer and developmental disorders. In cancer, aberrant expression of KMT5C has been observed in several tumor types, and its overexpression has been associated with poor prognosis. Mutations in the KMT5C gene have been linked to developmental disorders, such as intellectual disability and autism spectrum disorders. These findings highlight the importance of KMT5C in brain development and function, and provide new insights into the underlying mechanisms of these conditions.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Flow cytometry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
