| Field | Specification |
|---|---|
| Mfr No | |
| Form | 2X Mastermix |
| Product Type |
Kodaq 2X PCR MasterMix is a ready-to-use mixture containing Kodaq DNA Polymerase and all the necessary reagents for amplification of DNA in a uniquely formulated buffer with gel loading dye. Kodaq DNA Polymerase is a next-generation enzyme engineered for superior accuracy, robustness, and high-yield amplification, making it ideal for a wide range of PCR applications. This novel polymerase features strategically designed mutations that enhance processivity, DNA binding affinity, and stability, ensuring reliable and efficient performance. With exceptional 3’-5’ exonuclease activity, Kodaq offers unmatched fidelity compared to standard polymerases. Its advanced buffer system is optimized to tolerate high A/T and G/C content while overcoming common PCR inhibitors, including those present in plant-derived samples.
Product Features:
- High-fidelity PCR for superior accuracy
- Robust performance, resistant to common PCR inhibitors
- Reliable amplification of A/T- and G/C-rich templates
- Highly processive, delivering high-yield PCR results
- Included in abm's PCR Buffet Program
Please refer to the product page for component information.
| Specification | Value |
|---|---|
| Enzyme Type | PCR Mastermix |
| Format | 2X Mastermix |
| Hot Start | No |
| Fidelity | High-Fidelity |
| 3'→5' Proofreading | Yes |
This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
It contains Kodaq DNA Polymerase, an engineered high-fidelity enzyme with strong 3′ → 5′ proofreading activity, exceptional accuracy, and superior processivity, ensuring reliable results.
Kodaq has much higher accuracy due to its 3′ → 5′ exonuclease proofreading activity, reducing errors in amplified products.
Yes. The proprietary buffer is designed to tolerate high GC/AT content and many common PCR inhibitors, making it suitable for difficult templates.
Kodaq supports long-range PCR: up to 18 kb from easier targets or 15 kb from genomic DNA.
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