| Field | Specification |
|---|---|
| Target | |
| Alternative names | L-APB monohydrate |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C4H12NO6P |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
L-AP4 monohydrate, also known as L-APB monohydrate, is a potent, specific agonist of the group III mGluRs, with EC50 values of 0.13 μM, 0.29 μM, 1.0 μM and 249 μM for mGlu4, mGlu8, mGlu6 and mGlu7 receptors, respectively[1][2]. It is supplied as a white to off-white solid (C4H12NO6P, MW 201.11) at 99.0% purity.
Physical & Chemical Properties
| CAS Number | 2247534-79-6 |
|---|---|
| Molecular Formula | C4H12NO6P |
| Molecular Weight | 201.11 g/mol |
| Purity | 99.0% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | O=C(O)[C@@H](N)CCP(O)(O)=O.O |
| Target | mGlu, mGlu8, mGlu7 |
| Signaling Pathway | GPCR/G Protein; Neuronal Signaling |
| Solubility | In Vitro: DMSO: 10 mg/mL (49.72 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | 4°C, sealed storage, away from moisture and light. In solvent: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target[1]
|
mGlu4 0.13 μM (EC50) |
mGlu8 0.29 μM (EC50) |
mGlu6 1.0 μM (EC50) |
mGlu7 249 μM (EC50) |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 10 mg/mL (49.72 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); sealed storage, away from moisture and light; avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vivo
In eight nerve-ligated rats, the paw withdrawal threshold to von Frey filament application rises significantly, and does so dose-dependently, with L-AP4 (5-30 μg, intrathecal injection 4-5 days). No evident motor dysfunction accompanies intrathecal delivery of the various L-AP4 doses[2]. Paw withdrawal latency in these normal rats is not significantly changed by an intrathecal injection of L-AP4 at 30 μg[2]. When 5 to 50 μM L-AP4 was applied topically to the spinal cord, neuronal responses evoked by touch, pressure, pinch, and von Frey filaments were significantly inhibited, with the extent of inhibition depending on concentration[2].
| Animal Model | Rats.[2] |
|---|---|
| Dosage | 5-30 μg. |
| Administration | Intrathecal inhection 4-5 days. |
| Result | Dose-dependently increased paw withdrawal threshold. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
To quote quickly, tell us: compound name + CAS/structure (SMILES or mol file), intended assay context, solvent preference, salt/stereochemistry requirements, purity/QC level, and the amount (mg–g).
Can’t find the compound you’re looking for?
Send the CAS or structure and your specs. We can help source it, suggest close equivalents, or discuss custom synthesis with the right QC documentation (RUO).
Identification of 2-PMPA as a novel inhibitor of cytosolic carboxypeptidases. Biochem Biophys Res Commun 2020 Dec 17;533(4):1393-1399. PMID: 33092792
