| Field | Specification |
|---|---|
| Alternative names | NG-Nitroarginine methyl ester hydrochloride |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C7H16ClN5O4 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
L-NAME hydrochloride, also known as NG-Nitroarginine methyl ester hydrochloride, inhibits NOS with an IC50 of 70 μM. It is a precursor to the NOS inhibitor L-NOARG, which has an IC50 value of 1.4 μM, and it requires hydrolysis of its methyl ester by cellular esterases to become a fully functional inhibitor. It can be used to induce hypertension and preeclampsia models. It is supplied as a white to off-white solid (C7H16ClN5O4, MW 269.69) at 99.89% purity.
Physical & Chemical Properties
| CAS Number | 51298-62-5 |
|---|---|
| Molecular Formula | C7H16ClN5O4 |
| Molecular Weight | 269.69 g/mol |
| Purity | 99.89% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | N[C@@H](CCCNC(N[N+]([O-])=O)=N)C(OC)=O.[H]Cl |
| Signaling Pathway | Immunology/Inflammation |
| Solubility | In Vitro: H2O: 100 mg/mL (370.80 mM; Requires sonication) DMSO: 100 mg/mL (370.80 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | -20°C, sealed storage, away from moisture. In solvent: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target
IC50: 70 μM (NOS)[1]
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| H2O | 100 mg/mL (370.80 mM) | requires sonication |
| DMSO | 100 mg/mL (370.80 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); sealed storage, away from moisture; avoid repeated freeze-thaw cycles.
If water is used as the stock solvent, dilute to the working solution and sterilize it through a 0.22 μm filter before use.
In Vivo
Choose the formulation that suits the animal model and route of administration. Percentages are volume ratios of the final working solution. Prepare the working solution fresh on the day of dosing; if precipitation or phase separation occurs, gentle warming or sonication can help.
Direct preparation of the working solution
These formulations are prepared directly, without a DMSO stock; use them promptly after preparation.
Protocol 1
| Composition | PBS |
|---|---|
| Result | 140 mg/mL (519.11 mM); clear solution; requires sonication |
Data provided by the manufacturer.
In Vitro
L-arginine analogues are widely used as inhibitors of nitric oxide synthase (NOS) activity, and Nw-nitro-L-arginine methyl ester (L-NAME) is the leading example[2]. Against purified brain NOS, freshly dissolved L-NAME is a 50 fold weaker inhibitor (mean IC50= 70 μM) than L-NOARG (IC50= 1.4 μM), yet the apparent inhibitory potency of L-NAME approached that of L-NOARG after prolonged incubation at neutral or alkaline pH. HPLC analyses show a close correlation between hydrolysis of L-NAME to L-NOARG and NOS inhibition by L-NAME[1].
In Vivo
| Animal Model | Male Sprague-Dawley (SD) rats [7] |
|---|---|
| Dosage | 40 mg/kg, 5 weeks |
| Administration | drinking water |
| Result | Induced hypertension with body weight loss and high blood pressure. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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