| Field | Specification |
|---|---|
| Target | |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C8H19Cl2N3O2 |
| SMILES | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
L-NIL dihydrochloride is an inhibitor of inducible NO synthase, with an IC50 of 3.3 μM for miNOS[1][2][3]. It has a molecular formula of C8H19Cl2N3O2 and a molecular weight of 260.16 g/mol.
Physical & Chemical Properties
| CAS Number | 159190-45-1 |
|---|---|
| Molecular Formula | C8H19Cl2N3O2 |
| Molecular Weight | 260.16 g/mol |
| SMILES | N[C@@H](CCCCNC(C)=N)C(O)=O.[H]Cl.[H]Cl |
| Target | iNOS |
| Signaling Pathway | Immunology/Inflammation |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with your institution's chemical hygiene plan.
In Vitro
L-NIL concentration-dependently inhibits mouse inducible NOS (miNOS) as well as rat brain constitutive NOS (rcNOS), with considerably greater potency toward miNOS. With miNOS and rcNOS, L-NIL gives IC50 values of 3.3 and 92 pM, respectively, which indicates 28-fold selectivity for miNOS. L-NIL is also approximately 6-fold more potent against miNOS than either L-NMA or L-NNA[2].
In Vivo
In mice, renal IR-induced inflammation, oxidative stress and autophagy are prevented by L-NIL (10 and 30 mg/kg, IP)[1].
| Animal Model | Adult male Balb/c (20-25 g)[1]. |
|---|---|
| Dosage | 10 and 30 mg/kg. |
| Administration | Intraperitoneally at the end of CLP and at 6 h after sepsis induction. |
| Result | Led to a negligible increase in plasma NGAL compared to sham mice. Led to a significant decrease in both TLR4 and IL1βprotein contents and clusterin transcript. Showed an increase in NFAT5 mRNA levels, as compared with mice treated with vehicle. Promoted a decrease in AR protein expression, as compared with animals treated with vehicle. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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Activatable near-infrared probes for the detection of specific populations of tumour-infiltrating leukocytes in vivo and in urine. Nat Biomed Eng 2023 Mar;7(3):281-297. PMID: 36941352
Host glutathione is required for Rickettsia parkeri cell division and intracellular survival. Nat Commun 2025 Jul 1;16(1):5547. PMID: 40593553
iNOS contributes to heart failure with preserved ejection fraction through mitochondrial dysfunction and Akt S-nitrosylation. J Adv Res 2023 Jan:43:175-186. PMID: 36585107
SARS-CoV-2 induces neutrophil degranulation and differentiation into myeloid-derived suppressor cells associated with severe COVID-19. Sci Transl Med 2025 May 21;17(799):eadn7527. PMID: 40397714
Inducible nitric oxide synthase (iNOS)-activated Cxcr2 signaling in myeloid cells promotes TGFβ-dependent squamous cell carcinoma lung metastasis. Cancer Lett 2023 Aug 28:570:216330. PMID: 37524225
Cigarette smoke induces the ROS accumulation and iNOS activation through deactivation of Nrf-2/SIRT3 axis to mediate the human bronchial epithelium ferroptosis. Free Radic Biol Med 2023 May 1:200:73-86. PMID: 36871899
Quercetin Inhibits Neuronal Pyroptosis and Ferroptosis by Modulating Microglial M1/M2 Polarization in Atherosclerosis. J Agric Food Chem 2024 May 29;72(21):12156-12170. PMID: 38755521
INOS ablation promotes corneal wound healing via activation of Akt signaling. Exp Eye Res 2024 Jun:243:109886. PMID: 38583755
Exploring the role of iNOS in HFpEF-Related myocardial fibrosis: Involvement of PTEN-PI3K/AKT signaling pathway. Biochem Biophys Res Commun 2024 Nov 19:734:150589. PMID: 39245028
A genetic screen identifies O-antigen as essential for Rickettsia parkeri survival in macrophages by protecting from inflammasomes and interferon-stimulated genes. bioRxiv 2026 Jun 13:2026.06.12.731980. PMID: 42327330
