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The L6 cell line is a well-established model derived from rat skeletal muscle tissue. These cells are notable for their ability to differentiate into myotubes, making them a valuable tool for studying muscle development, regeneration, and physiology. L6 cells exhibit robust proliferative capacity and are commonly used in research focusing on muscle cell biology, including studies on muscle protein synthesis, hypertrophy, and atrophy. The differentiation process in L6 cells can be induced under specific culture conditions, leading to the formation of multinucleated myotubes that closely mimic the characteristics of mature skeletal muscle fibers.
In addition to their applications in muscle physiology research, L6 cells are also employed in metabolic studies, particularly those involving glucose uptake and insulin signaling pathways. These cells express insulin receptors and can be used to investigate the molecular mechanisms underlying insulin resistance and diabetes. The L6 cell line's responsiveness to various metabolic stimuli makes it an ideal model for exploring the effects of different treatments or genetic modifications on muscle metabolism. Overall, L6 cells provide a versatile and reliable platform for advancing our understanding of muscle biology and metabolic diseases.
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Protein expression: myosin
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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