| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ladinin-1; Lad-1; Linear IgA disease antigen (LADA); LAD1; LAD |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic peptide, corresponding to amino acids 218-260 of Human Lad. |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Lad (TSAD, TSAd, F2771, SH2D2A, SH2 domain protein 2A) is a 52 kDa cytoplasmic adapter protein that undergoes tyrosine-phosphorylation and influences T cell activation. Lad (SH2D2A) mRNA is present in peripheral blood leukocytes, thymus and spleen, and accumulates in the cytoplasm during T cell activation. The Lad gene maps to chromosome 1q21 in a region where alterations are characteristic to lymphomas.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes Lad. Lad (I249) polyclonal antibody detects endogenous levels of Lad protein.
Validated Applications
| Applications | Western Blot, Immunohistochemistry |
|---|---|
| WB | 1:500-1:1000 |
| IHC | 1:50-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Synthetic peptide, corresponding to amino acids 218-260 of Human Lad.
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Immunogen | Synthetic peptide, corresponding to amino acids 218-260 of Human Lad. |
| Purity | > 95% (by SDS-PAGE) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | Rabbit IgG, 1 mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Target MW | ~ 54 kDa |
| Swiss-Prot | O00515 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: Human. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Lad research from Bioworld Technology Inc, or contact us for alternative formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
