| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Puromycin + Blasticidin |
| Shipping | |
| Species |
Background
LAG3 (lymphocyte-activation gene 3) is a co-inhibitory immune checkpoint receptor expressed on activated T cells, regulatory T cells, and other lymphocytes. By binding ligands such as MHC class II and fibrinogen-like protein 1, LAG3 transmits inhibitory signals that dampen T cell activation, proliferation, and cytokine production, contributing to peripheral tolerance and limiting excessive immune responses. In tumors, sustained LAG3 expression marks exhausted T cells and supports immune evasion. LAG3 is a validated immunotherapy target, and antibodies that block LAG3 are being developed to restore antitumor T cell function, often in combination with other checkpoint inhibitors.
Product Description & Applications
The LAG3/IL-2 Reporter Lentivirus Set is a two-component immunotherapy reporter system for studying the LAG3 immune checkpoint. One lentivirus constitutively expresses the human LAG3 receptor; the second carries an IL-2-responsive dual reporter combining Gaussia luciferase with a fluorescent protein (eGFP or mCherry). Ligand engagement of LAG3 suppresses IL-2-driven reporter expression, while checkpoint inhibitors that block LAG3 restore the signal, providing a quantitative readout of checkpoint blockade. Sequential transduction with puromycin and blasticidin selection generates a dual-stable effector cell line. The system is used to evaluate LAG3-targeting antibodies and to monitor T cell activation in immuno-oncology research. Supplied as high-titer, VSV-G pseudotyped lentiviral particles suitable for difficult-to-transfect cells, including primary and thawed cells.
About This Product
This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human LAG3 under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (Gaussia Luciferase + eGFP, Gaussia Luciferase + mCherry). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.
Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
