Lama-84 cell

SKU:BHC11100572
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Overview
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Lama-84 cell is a cell line derived from Caucasian (Female). It is commonly used as an in vitro model for 1 research. Growth characteristics: Suspension, some adherent cells, Round cells. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Disease model Chronic myeloid leukemia
Morphology Round cells
Growth Properties Suspension, some adherent cells
Tissue Blood
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Catalog no. Size
300261 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 300261
Species Human
LAMA-84 is a human cell line derived from the peripheral blood of a patient with chronic myeloid leukemia (CML) in blast crisis. This cell line is characterized by the presence of the Philadelphia chromosome, which results in the BCR-ABL fusion gene, a hallmark of CML. The BCR-ABL oncogene is known for its role in increasing tyrosine kinase activity, which promotes various signaling pathways leading to uncontrolled cell proliferation and resistance to apoptosis. LAMA-84 cells are thus an invaluable model for studying the molecular mechanisms of CML progression and for evaluating the efficacy of tyrosine kinase inhibitors (TKIs) in a pre-clinical setting. In research, LAMA-84 has been extensively used to understand the biology of CML, especially in the context of drug resistance and disease evolution. Studies involving this cell line have helped in elucidating the cellular responses to different generations of TKIs, such as imatinib, dasatinib, and nilotinib. Moreover, LAMA-84 has contributed to the investigation of new therapeutic strategies aimed at overcoming TKI resistance, including the testing of combination therapies that target other signaling pathways synergistically affected by the BCR-ABL fusion protein.

SKU:BHC11100572

  • Surface antigens: GPIIb/IIIa+, GPIIIa+
  • Viruses: EBNA, EA, and VCA were not detected
  • Mutational profile: BCR-ABL1 pos
  • cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
  • supplements: Supplement the medium with 10% heat-inactivated FBS
  • doublingTime: 30 hours
  • subculturing: Cells adhering to the bottom of the cell culture flask may be dislodged by shaking. Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 105 cells/ml and keep the cell concentration within the range of 3 x 105 to 1 x 106 cells/ml for optimal growth.
  • seedingDensity: 1 to 2 x 104 cells/cm2
  • postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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