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Description
The LAMP Probe Assay Kit (Bst Plus HSS) is a reagent designed for LAMP amplification using DNA as a template. It utilizes the next-generation Bst DNA Polymerase combined with an optimized buffer system to achieve highly sensitive amplification with excellent specificity. The buffer in this product does not contain nucleic acid dyes, making it suitable for probe-based detection. For fluorescence-based methods, additional dye needs to be added.
Component
|
Component Number |
Component Name |
16721ES60 (100T) |
|
16721-A |
4×Bst Buffer |
600 µL |
|
16721-B |
Bst Plus HSS DNA Polymerase (40 U/µL) |
100 µL |
Storage Conditions
Store at -25°C to -15°C. Shelf life: 1 year. If not used for an extended period, store protected from light.
Instructions for Use
1. Reaction System
|
Component |
Volume (µL) |
Final Concentration |
|
4×Bst Buffer |
6 |
1× |
|
Bst Plus HSS DNA Polymerase (8U/µL) |
1 |
- |
|
Primer mix* |
2.5 |
- |
|
Template |
1-10 |
- |
|
ddH2O |
up to 25 |
- |
*Primer mix: Recommended primer concentrations are 16 µM FIP/BIP, 2 µM F3/B3, and 4 µM LoopF/LoopB. The probe concentration should be optimized based on the design. Other primer set ratios may also be tested for optimal performance.
2. Reaction Conditions
Incubate at 63-65°C for 30-60 minutes, followed by enzyme inactivation at 85°C for 5 minutes.
Note
For your safety and health, please wear a lab coat and disposable gloves while handling the reagents.
RT-LAMP and LAMP reactions in this kit proceed isothermally at 60–65°C (optimized temperature specified in the datasheet). A standard heat block, water bath, or isothermal dry-bath incubator is sufficient — no thermocycler is required. This makes the kit well-suited to resource-limited or point-of-care settings where conventional PCR instrumentation is unavailable.
The Hotstart Bst Plus enzyme used in this kit incorporates an antibody-mediated cold-block that prevents non-specific amplification and false-positive results at ambient temperature. Activity is released upon incubation at ≥60°C, improving assay specificity and enabling pre-reaction setup at room temperature without risk of non-specific product formation.
Detection method depends on the kit format: Dye Assay kits use intercalating fluorescent dyes (e.g., SYBR Green, calcein, or YSFluor) for visual or instrument-based detection; Fluorescent Assay kits use real-time fluorescence monitoring; Probe Assay kits use sequence-specific probes for enhanced specificity. Refer to the product title and datasheet for the exact detection format in the kit you ordered.
This kit is validated for purified RNA or DNA templates. Some formats support direct amplification from swabs, saliva, or crude lysates without prior nucleic acid extraction — check the specific product datasheet for the validated sample input types, inhibitor tolerances, and any pre-treatment requirements.
LAMP amplification typically completes within 20–60 minutes at the isothermal incubation temperature, compared to 1.5–3 hours for conventional RT-PCR. The faster time-to-result makes this kit especially useful for high-throughput screening workflows and time-sensitive applications such as outbreak response and quarantine protocols.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
