| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | FEX antibody; G protein coupled receptor 49 antibody; G protein coupled receptor 67 antibody; g protein-coupled receptor fex antibody; G-protein coupled receptor 49 antibody; G-protein coupled receptor 67 antibody; G-protein coupled receptor HG38 antibody; GPCR GPR49 antibody; GPR 49 antibody; GPR 67 antibody; GPR49 antibody; GPR67 antibody; GRP 49 antibody; GRP49 antibody; HG 38 antibody; HG38 antibody; Leucine rich repeat containing G protein coupled receptor 5 antibody; Leucine-rich repeat-containing G-protein coupled receptor 5 antibody; LGR 5 antibody; LGR5 antibody; LGR5_HUMAN antibody; MGC117008 antibody; Orphan G protein coupled receptor HG38 antibody |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human LGR5 protein |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a recombinant monoclonal antibody expressed in HEK293F cells. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a monoclonal anti-LGR5 antibody raised in Human, with confirmed utility in ELISA. It is designed to detect LGR5 protein in Human and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Recombinant Human LGR5 protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Human): Requires anti-human-IgG secondary reagents for indirect detection.
- Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
- Isotype (IgG2): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Affinity chromatography): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
LGR5 (also referred to as FEX antibody, G protein coupled receptor 49 antibody, G protein coupled receptor 67 antibody, g protein-coupled receptor fex antibody, G-protein coupled receptor 49 antibody, G-protein coupled receptor 67 antibody, G-protein coupled receptor HG38 antibody, GPCR GPR49 antibody, GPR 49 antibody, GPR 67 antibody, GPR49 antibody, GPR67 antibody, GRP 49 antibody, GRP49 antibody, HG 38 antibody, HG38 antibody, Leucine rich repeat containing G protein coupled receptor 5 antibody, Leucine-rich repeat-containing G-protein coupled receptor 5 antibody, LGR 5 antibody, LGR5 antibody, LGR5_HUMAN antibody, MGC117008 antibody, Orphan G protein coupled receptor HG38 antibody) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of LGR5 biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG2 from Human) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a recombinant monoclonal antibody expressed in HEK293F cells.
LGR5 is a protein target in Human biology. This monoclonal antibody raised in Human is designed to detect LGR5 in ELISA applications, with IgG2 isotype.
Reported reactive against Human. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG2 antibody requires a compatible secondary. Select anti-human IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG2.
(1) Isotype control — IgG2 from Human at matching concentration; (2) Positive control — known LGR5-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
