| Field | Specification |
|---|---|
| Alternative Names | ligA; DP1006; DNA ligase; EC 6.5.1.2; Polydeoxyribonucleotide synthase [NAD(+)] |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Desulfotalea psychrophila DNA ligase protein (340-679AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-ligA antibody raised in Rabbit, conjugated with HRP, with confirmed utility in ELISA. It is designed to detect ligA protein in Desulfotalea psychrophila and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Recombinant Desulfotalea psychrophila DNA ligase protein (340-679AA) — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Protein G purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
- HRP conjugate: Direct detection with TMB/OPD or ECL substrates. Quench endogenous peroxidase before use in tissue assays.
Biological background
ligA (also referred to as ligA, DP1006, DNA ligase, EC 6.5.1.2, Polydeoxyribonucleotide synthase [NAD(+)]) is a protein target studied in Desulfotalea psychrophila systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of ligA biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Desulfotalea psychrophila. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
ligA is a protein target in Desulfotalea psychrophila biology. This polyclonal antibody raised in Rabbit is designed to detect ligA in ELISA applications, with IgG isotype.
Reported reactive against Desulfotalea psychrophila. Immunogen derived from Desulfotalea psychrophila. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
HRP enables direct TMB/ECL detection without secondary. Quench endogenous peroxidase in tissue assays.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known ligA-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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