Liposome Drug Dissolution Assay Kit

SKU:BHT16300162
Overview
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Liposome Drug Dissolution Assay Kit for in vitro release testing of liposomal drugs, using an ammonium sulfate dissolution buffer and spin columns that remove released drug. Remaining encapsulated drug is read by absorbance in 96-well plates (490 nm for doxorubicin). One size: 5 dissolution tests.
Assay Target Liposomal drug dissolution
Detection Method Colorimetric
Detection Wavelength Absorbance 490 nm (doxorubicin)
Plate Format Spin columns + 96-well plate
Options selector
Catalog no. Size
LDD05 5 assays
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 5 assays
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Please contact us to confirm storage conditions.
  • Shipping: Shipped cold (not frozen).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Applications
  • Drug Delivery
Assay target Liposomal drug dissolution
Assay area Drug Delivery
Detection method
  • Colorimetric
Detection wavelength Absorbance 490 nm (doxorubicin)
Instrument required Centrifuge + absorbance reader
Plate format Spin columns + 96-well plate
Storage Please contact us to confirm storage conditions.
Shipping Shipped cold (not frozen).
Catalog no. (Mfr.) LDD05
Main SKU BHT16300162
Liposome Assay Kit

Liposome drug dissolution is the release of an encapsulated drug into the liposome's surrounding medium. In vitro dissolution tests apply chemical and physical stress to simulate this release; for liposomes loaded by pH-gradient remote loading, an ammonium salt is added to speed up release.

Kit Components

Component LDD05
Prepacked spin columns 40 columns
10 x Dissolution buffer 1.5 ml
Elution buffer 10 ml

10 x Dissolution buffer: 2 M (NH4)2SO4, pH 5.5. Elution buffer: 10 mM histidine, pH 6.5, 9.2 % sucrose.

Assay Principle

Liposomal drug is incubated in dissolution buffer at a set temperature; at each time point the released drug is removed on a spin column and the drug remaining in intact liposomes is measured by absorbance. Dissolution (%) is calculated against the time-zero sample, and a half-life is obtained by curve fitting.

Specifications

Assay Target Liposomal drug dissolution
Detection Method Colorimetric
Detection Wavelength Absorbance 490 nm (doxorubicin)
Plate Format Spin columns + 96-well plate
Instrument Required Centrifuge + absorbance reader
Size 5 assays
Storage Please contact us to confirm storage conditions.
Shipping Shipped cold (not frozen).

Protocol

The manufacturer's protocol describes the procedure and reaction volumes.

  • Liposome Drug Dissolution Assay (PDF, see Documents): Covers catalog # LDD05.

Safety & Handling

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The Liposome Drug Dissolution Assay Kit is shipped cold (not frozen). Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.
Q.What does this kit measure?
A.It measures how fast an encapsulated drug is released from liposomes over time at a chosen temperature, giving % dissolution and a release half-life.
Q.What is included in the kit?
A.The Liposome Drug Dissolution Assay Kit (5 assays) includes prepacked spin columns (40 columns), 10 x Dissolution buffer (1.5 ml) and elution buffer (10 ml).
Q.Is a protocol available?
A.Yes. The manufacturer's protocol (Liposome Drug Dissolution Assay, linked under Documents) covers catalog # LDD05.
Q.How should the kit be stored?
A.The Liposome Drug Dissolution Assay Kit is shipped cold (not frozen). Please contact us for long-term storage conditions.

Can’t find the exact format you need? We can help you source or customize this liposomal drug dissolution assay — including larger pack sizes, bulk reagents, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.

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Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today