| Field | Specification |
|---|---|
| Applications | |
| Assay target | |
| Assay area | |
| Detection method | |
| Instrument required | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Liposomal drug samples can contain free drug that is not inside the liposomes. Drug may leak during storage or after exposure to organic solvent, ultrasound, freezing or heat, and drug loading during production may be incomplete.
Kit Components
| Component | LDE10 |
|---|---|
| Prepacked spin columns | 30 columns |
| Elution buffer | 10 ml |
Enough for 10 liposome samples measured in triplicate (3 columns per sample).
Assay Principle
Spin columns separate intact liposomes from non-encapsulated drug based on differences between liposomes and free drug molecules, including size. The absorbance of the liposome eluate is compared with the total (unseparated) sample to give the encapsulation yield (%).
Specifications
| Assay Target | Liposomal drug encapsulation (%) |
|---|---|
| Detection Method | Colorimetric |
| Instrument Required | Centrifuge + absorbance reader |
| Liposome Recovery | 100 % ± 2 % |
| Free Drug Removal | > 98 % |
| Size | 10 assays |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped cold (not frozen). |
Protocol
The manufacturer's protocol describes the procedure and reaction volumes.
- Liposome Drug Encapsulation Assay (PDF, see Documents): Covers catalog # LDE10.
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this liposomal drug encapsulation (%) assay — including larger pack sizes, bulk reagents, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.
