| Field | Specification |
|---|---|
| Applications | |
| Assay type | |
| Assay target | |
| Assay area | |
| Instrument required | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
The kit tests how stable liposomal drugs stay in plasma or serum from humans or animals. Its ready-to-use spin columns separate intact liposomes from released drug and plasma proteins, so the fraction of drug still held in intact liposomes can be measured.
Kit Components
| Component | SPS20 |
|---|---|
| Prepacked spin columns | 20 columns |
The kit contains only the 20 prepacked spin columns (one column per sample). Plasma or serum, a 0.22 µm low-protein-binding syringe filter, 1.5 ml receiver tubes and the method used to measure the drug in the eluate are not included.
Assay Principle
After incubation with plasma, the sample is spun through a prepacked spin column. More than 95 % of plasma proteins, together with free and protein-bound drug, stay on the column, while intact liposomal drug passes into the eluate. Recovery of intact liposome is the drug concentration in the eluate of the plasma-treated sample divided by that of a buffer control kept on ice. In the manufacturer's example, liposomal doxorubicin gave 96 % recovery after 2 hours in human plasma at 37 °C.
Specifications
| Assay Target | Liposome stability in plasma |
|---|---|
| Assay Type | Stability Assay |
| Instrument Required | Benchtop microcentrifuge |
| Samples per Kit | 20 (one spin column per sample) |
| Sample Type | Liposomal drugs in human or animal plasma or serum |
| Not Included | Plasma or serum, 0.22 µm syringe filter, receiver tubes and drug detection reagents |
| Size | 20 columns |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped cold (not frozen). |
Protocol
The manufacturer's protocol describes the procedure and reaction volumes.
- Liposome Plasma Stability Test (PDF, see Documents): Covers catalog # SPS20.
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this liposome stability in plasma assay — including larger pack sizes, bulk reagents, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.
