| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C42H71NO7 |
| Purity | |
| SMILES | |
| Form | Liquid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
LNP Lipid-8 (11-A-M) is an ionizable single-tail, multi-head lipid that can be used to form a lipid nanoparticle (LNP) that delivers siRNA to T cells without targeting ligands, showing greater selectivity for T cells than for other cell types such as hepatocytes. It selectively delivers siRNA/sgRNA to T cells, especially CD8+ T cells, through endogenous lipid transport pathways and can enter cells and release RNA via endocytosis, achieving gene silencing; when loaded with GFP siRNA (siGFP), it produced significant GFP gene silencing in mouse models. It showed good efficacy and safety in both cells and animals, without evident liver targeting or toxicity, and can be applied to RNA delivery research in tumor immunotherapy and T cell-mediated autoimmune diseases[1]. It is a colorless to light yellow liquid (C42H71NO7, MW 702.02) at 95.0% purity.
Physical & Chemical Properties
| CAS Number | 2408140-60-1 |
|---|---|
| Molecular Formula | C42H71NO7 |
| Molecular Weight | 702.02 g/mol |
| Purity | 95.0% |
| Appearance | Liquid |
| Color | Colorless to light yellow |
| Density | 1.034±0.06 g/cm3 |
| SMILES | CCN(CC)CCCOC(OCC(COC(CCCCCCC/C=C\C/C=C\CCCCC)=O)COC(C[C@@]12CC3C[C@H](C[C@@H](C3)C2)C1)=O)=O |
| Signaling Pathway | Metabolic Enzyme/Protease |
| Solubility | In Vitro: DMSO: ≥ 100 mg/mL (142.45 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) * "≥" means soluble, but saturation unknown. |
| Storage | Pure form: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | ≥ 100 mg/mL (142.45 mM) | use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | ≥ 2.5 mg/mL (3.56 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.5 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Protocol 2
| Composition | 10% DMSO + 90% (20% SBE-β-CD in saline) |
|---|---|
| Result | 2.5 mg/mL (3.56 mM); suspension; requires sonication |
| How to prepare | Gives a suspension at 2.5 mg/mL. The suspension is suitable for oral and intraperitoneal dosing. For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 900 μL 20% SBE-β-CD in saline. 20% SBE-β-CD in saline: dissolve 2 g SBE-β-CD powder in 10 mL saline until clear (4°C, store up to one week). |
Protocol 3
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | ≥ 2.5 mg/mL (3.56 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.5 mg/mL (saturation not determined). Use with caution if continuous dosing will exceed two weeks. For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 900 μL corn oil. |
Data provided by the manufacturer.
In Vitro
In vitro, LNP Lipid-8 can encapsulate about 75% of siRNA/sgRNA, and transmission electron microscopy reveals a spherical structure[1].
In Vivo
In splenic CD3+ T cells of a GFP transgenic C57BL/6 mouse model, GFP expression is significantly reduced by GFP-siRNA (siGFP) loaded in LNP Lipid-8 (1.5 mg/kg; intravenous injection; single dose; 3 days), and CD8+ T cells show higher silencing efficiency than CD4+ T cells[1]. T cell gene silencing is achieved in a GFP transgenic C57BL/6 mouse model by siGFP loaded in LNP Lipid-8 (0.5 mg/kg; intravenous injection; single dose; 3 days); the lowest effective dose is 0.5 mg/kg, and liver toxicity is not obvious[1]. Delivery of sgRNA in a Cas9-GFP transgenic mouse model was achieved with siGFP-loaded LNP Lipid-8 (2.0 mg/kg; intravenous injection; single dose; 5 days), which induced GFP gene editing in CD8+ T cells; the editing efficiency was significantly higher there than in CD4+ T cells[1].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
To quote quickly, tell us: compound name + CAS/structure (SMILES or mol file), intended assay context, solvent preference, salt/stereochemistry requirements, purity/QC level, and the amount (mg–g).
Can’t find the compound you’re looking for?
Send the CAS or structure and your specs. We can help source it, suggest close equivalents, or discuss custom synthesis with the right QC documentation (RUO).
