| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human C1orf92/LRRC71 recombinant protein (Position: K35-D543) was used as the immunogen for the LRRC71 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LRRC71 Antibody / Leucine-rich repeat-containing protein 71 is a anti-LRRC71 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: LRRC71
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
LRRC71 is encoded by the LRRC71 gene located on human chromosome 8q24.3. The protein contains tandem LRR motifs forming a horseshoe-shaped structure that facilitates binding to various partners, including receptors, kinases, and cytoskeletal proteins. Bioinformatic analyses suggest roles in cell differentiation and communication, possibly through modulation of receptor signaling. Expression profiling has revealed LRRC71 mRNA in multiple tissues, with enrichment in testis, brain, and epithelial organs, indicating possible participation in spermatogenesis and neurodevelopment.
Using the LRRC71 antibody, researchers can detect a band between 60-70 kilodaltons on western blot, corresponding to the predicted molecular weight of the protein. Immunostaining studies show cytoplasmic and perinuclear localization, consistent with a signaling or adaptor function. Although direct physiological functions remain under active investigation, related LRR family members are known to mediate receptor clustering and intracellular scaffolding, suggesting a conserved molecular role for LRRC71 in signal integration and cell organization.
Emerging data suggest LRRC71 may interact with intracellular components regulating calcium signaling or vesicular trafficking. Its predicted structural features imply involvement in membrane-proximal signaling complexes that respond to developmental or stress cues. Further investigation may also link LRRC71 to fertility regulation, as several LRR proteins participate in germ cell communication and acrosome formation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
