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Scientific Background
This ELISA kit applies to the in vitro quantitative determination of LXA4 concentrations in serum, plasma and other biological fluids.
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal LXA4. During the reaction, Universal LXA4 in the sample or standard competes with a fixed amount of Universal LXA4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal LXA4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal LXA4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Performance Specifications
| Sensitivity | 0.47 ng/mL |
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| Detection Range | 0.78-50 ng/mL |
| Total Assay Time | 2 h 30 min |
| Compatible Sample Types | Serum, plasma and other biological fluids |
| Species Reactivity | Universal (multi-species validated) |
| Detection Method | Competitive |
| Precision (CV) | Both intra-CV and inter-CV are < 10%. |
| Recovery Rate | 80%-120% |
| Storage | 2-8℃,12 months |
✓ Research-Grade Validation
Specificity
This kit recognizes Universal LXA4 in samples.No significant cross-reactivity or interference between Universal LXA4 and analogues was observed
Safety & Regulatory
Handle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.
This kit is validated for use with Serum, plasma and other biological fluids. For unlisted matrices (e.g., tissue lysate, urine), perform a spike-and-recovery experiment to confirm assay performance before generating reportable data. Sample dilution in the kit's provided diluent is recommended to minimize matrix interference.
The minimum detectable concentration (sensitivity) of this kit is 0.47 ng/mL. Values below this threshold should be reported as below the limit of detection (<LOD) and should not be extrapolated from the standard curve.
The total assay time from sample addition to absorbance reading is approximately 2 h 30 min, including all incubation, wash, and substrate steps. Hands-on time is typically 1–2 hours; most steps involve passive plate incubation. Plan the assay as a single uninterrupted session for best results.
Standard components of this Competitive ELISA Kit typically include: pre-coated microplate (96-well strip format), lyophilized or liquid recombinant LXA4 standard, detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and sample/standard diluent. Refer to the kit insert or datasheet for the exact component list and storage requirements.
This kit uses colorimetric (TMB/HRP) detection and requires a standard microplate absorbance reader capable of measuring at 450 nm. A reference wavelength of 570 nm or 630 nm is recommended to reduce background. No specialized fluorescence or luminescence reader is needed. Ensure the instrument is calibrated and the plate is clean and free of condensation before reading.
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
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HPGD induces ferroptosis and autophagy to suppress esophageal squamous cell carcinoma through the LXA4–ERK1/2–U2AF2–TFRC axis (2025) Molecular Cancer. 10.1186/s12943-025-02464-x
P2X7-CaMKII drives 5-LOX nuclear translocation to impair microglial function after subarachnoid hemorrhage (2025) Journal of Neuroinflammation. 10.1186/s12974-025-03530-3
Genetically Engineered Macrophages Co-loaded with CD47 Inhibitors Synergistically Reconstruct Efferocytosis and Improve Cardiac Remodeling Post Myocardial Ischemia Reperfusion Injury (2024) Advanced Healthcare Materials. 10.1002/adhm.202303267
Targeted delivery and ROS-responsive release of Resolvin D1 by platelet chimeric liposome ameliorates myocardial ischemia–reperfusion injury (2022) JOURNAL OF NANOBIOTECHNOLOGY. 10.1186/s12951-022-01652-x
Pig lung fibrosis is active in the subacute CdCl2 exposure model and exerts cumulative toxicity through the M1/M2 imbalance (2021) ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY. 10.1016/j.ecoenv.2021.112757
LXA4 enhances prostate cancer progression by facilitating M2 macrophage polarization via inhibition of METTL3 (2022) INTERNATIONAL IMMUNOPHARMACOLOGY. 10.1016/j.intimp.2022.108586
Infective Larvae of Brugia malayi Induce Polarization of Host Macrophages that Helps in Immune Evasion (2018) Frontiers in Immunology. 10.3389/fimmu.2018.00194
The protective effects of lipoxin A4 on type 2 diabetes mellitus: A Chinese prospective cohort study (2023) Frontiers in Endocrinology. 10.3389/fendo.2023.1109747
WTAP-m6A-ALOX15 axis mediates dendritic cells-keratinocytes interaction involved in lipid metabolism disorders to drive atopic dermatitis (2025) LIFE SCIENCES. 10.1016/j.lfs.2025.124110
Lipoxin A4 activates ALX/FPR2 to attenuate inflammation in Aspergillus fumigatus keratitis (2021) INTERNATIONAL IMMUNOPHARMACOLOGY. 10.1016/j.intimp.2021.107785
Cadmium exposure induces TNF-α-mediated necroptosis via FPR2/TGF-β/NF-κB pathway in swine myocardium (2021) TOXICOLOGY. 10.1016/j.tox.2021.152733
Salivary levels of specialized pro-resolving lipid mediators as indicators of periodontal health/disease status (2019) JOURNAL OF CLINICAL PERIODONTOLOGY. 10.1111/jcpe.13173
Sirtuin6 and Lipoxin A4 levels are decreased in severe periodontitis (2023) Clinical Oral Investigations. 10.1007/s00784-023-05330-6
The Importance of Resolvin D1, LXA4, and LTB4 in Patients with Acute Pancreatitis Due to Gallstones (2025) Medicina-Lithuania. 10.3390/medicina61020239
