| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
The liver X receptor response element (LXRE) is a DNA motif bound by liver X receptors (LXR-alpha, encoded by NR1H3, and LXR-beta), which are ligand-activated nuclear receptors that function as master regulators of cholesterol and lipid metabolism. Activated by oxysterols and other cholesterol-derived ligands, LXRs heterodimerize with retinoid X receptors and bind LXREs to induce genes controlling cholesterol efflux, reverse cholesterol transport, fatty acid synthesis, and bile acid metabolism. LXRs also exert anti-inflammatory effects in macrophages. Their central roles in lipid homeostasis make LXR signaling an important target in research on atherosclerosis, metabolic disease, and inflammation.
Product Description & Applications
The LXRE Reporter Lentivirus places a reporter gene under the control of tandem liver X receptor response elements, providing a sensitive readout of LXR transcriptional activity in human and mouse cells. Reporter options include fluorescent (GFP, RFP) and luminescent (firefly and Renilla luciferase) formats, with blasticidin or puromycin selection for stable reporter cell line establishment. Signal can be measured by fluorescence microscopy, flow cytometry, or luminometry.
The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring LXR activation, studying cholesterol and lipid metabolism, and screening LXR agonists and antagonists.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the LXR pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
