LY 294002

Product details

• Chemical name: 2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one hydrochloride.
• CAS number: 154447-36-6
• Molecular formula: C19H17NO3.
• Purity: >99%
• Concentration: 5-10 µM.
• Form: Lyophilized powder.
• Solubility: DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
• Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.
• Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
• Target: PI3-Kinase
• Source: Synthetic
• Activity: LY 294002 is a potent and specific cell-permeable inhibitor of Pl3-kinase1.

Scientific background

PI 3-kinase is an enzyme implicated in growth factor signal transduction by associating with receptor and non-receptor tyrosine kinases.1,2 LY-294002 is a potent and specific cell-permeable inhibitor of Pl 3-kinase that acts on the ATP-binding site of the enzyme.3In in vitro assays it displays an IC50 of 1.4 µM on purified PI 3-kinase, but did not inhibit PKC, PKA, MAP kinase and PI 4-kinase.3 In fMet-Leu-Phe stimulated neutrophils, LY-294002 completely abolished PI 3-kinase activity at 50 µM.4 LY-294002 induces apoptosis in many cell types by blocking the PI3-K/Akt anti-apoptotic pathway.5,6 Thus LY-294002 is useful tool for identifying the biochemical events regulated by the PI3-K/Akt pathway.7 This pathway has important relevance to the cellular events involved in cancer.6,8,9

Lead time: 1-2 Business Days

Country of origin: Israel/IL

Applications key

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Bioassay tested: Yes

• SDS (PDF)

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• Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
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