| Field | Specification |
|---|---|
| Target | |
| Alternative names | KARS;CMTRIB;DFNB89;KARS1;KARS2;KRS |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant fusion protein of human LysRS/LysRS/KARS(NP_005539.1). |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes LysRS/KARS. Polyclonal Antibodies
Validated Applications
| Applications | Western Blot, Immunofluorescence |
|---|---|
| WB | 1:500-1:2000 |
| IF/ICC | 1:50-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Recombinant fusion protein of human LysRS/LysRS/KARS(NP_005539.1).
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | Recombinant fusion protein of human LysRS/LysRS/KARS(NP_005539.1). |
| Purity | > 95% (by SDS-PAGE) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | 1 mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Target MW | ~ 75 kDa |
| Swiss-Prot | Q15046 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for LysRS/KARS research from Bioworld Technology Inc, or contact us for alternative formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
