| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Lyticase is a specialized enzyme preparation for hydrolyzing yeast cell walls, widely used in downstream applications such as genome research, yeast artificial chromosome (YAC) extraction, yeast transformation, protoplast preparation, yeast cell disruption, industrial fermentation, and yeast inactivation.
This product is supplied in liquid form at a concentration of 10 U/μL, with a typical working concentration of 0.15 U/μL. The optimal concentration should be adjusted according to your specific application, yeast strain, and cell density. For genomic DNA or plasmid DNA extraction, please follow the recommended concentration in the corresponding kit protocol.
Features
Broad-Spectrum: Lyses a wide range of fungal cells for versatile applications.
Stable & Reliable: Consistent enzyme activity with easy storage and transport.
High-Efficiency: Rapid nucleic acid purification and high-quality protoplast generation.
Components
|
Name |
10403ES81 |
10403ES88 |
10403ES92 |
|
Lyticase (10 U/μL) |
1500 U |
3000 U |
10000 U |
Storage
This Product should be stored at -25°C ~ -15°C. Valid for 2 years.
Application
- Yeast cell wall digestion
- Protoplast preparation from fungi
- Yeast transformation and genetic manipulation
- Extraction of genomic DNA or plasmid DNA from yeast
- Preparation of high-quality fungal RNA
- Industrial fermentation and yeast inactivation
- Fungal biotechnology research
Glycerol Content: Contains Glycerol
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in NGS Library Preparation applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
Activity is expressed in lysate units (LU) or U/mg, defined as the amount of enzyme that produces a defined reduction in optical density (OD₆₀₀) of a bacterial suspension per minute under standard assay conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Lytic enzymes such as lysozyme cleave the β-1,4-glycosidic linkage between N-acetylmuramic acid and N-acetylglucosamine in bacterial peptidoglycan cell walls, releasing intracellular contents for downstream nucleic acid or protein extraction. Mutanolysin targets β-1,4-glycosidic bonds in the muramidase substrate of Gram-positive bacteria resistant to lysozyme. Enzyme lysis is typically gentler than mechanical disruption and reduces shear-induced DNA fragmentation.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
- Lu H, Zhu J, Zhang T, et al. A rapid multiplex nucleic acid detection system of airborne fungi by an integrated DNA release device and microfluidic chip. Talanta. 2022;246:123467. doi:10.1016/j.talanta.2022.123467(IF:6.057)
- Liao X, Zhao J, Liang S, et al. Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in Pichia pastoris. Biotechnol Biofuels. 2019;12:38. Published 2019 Feb 21. doi:10.1186/s13068-019-1377-z(IF:5.452)
