| Field | Specification |
|---|---|
| Applications | |
| Storage | |
| Shelf life | |
| Catalog no. (Mfr.) | |
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Product Overview
This kit is designed for purifying and enriching DNA and RNA from pathogenic microorganisms, such as viruses, bacteria, and fungi, present in biological fluid samples like sputum, bronchoalveolar lavage fluid, blood, and ascites. The purified microbial DNA and RNA are suitable for a variety of downstream applications, including PCR, RT-PCR, qPCR, NGS, and other molecular biology experiments.
The protocol mixes 400 µL of sample with 200 µL of Buffer LBS-A and 20 µL of Proteinase K, vortexed for 10 min or thermomixed at 65°C and 2500 rpm for 10 min. A second digestion with 250 µL of Buffer ML and 20 µL of Proteinase K runs at 56°C for 10 min, followed by clarification at 12,000 rpm for 1 min. Nucleic acids are bound with 400 µL of isopropanol and 30 µL of Magbeads PN at 1500 rpm for 3 min, washed twice with 800 µL of Buffer GW1 and twice with 800 µL of Buffer GW2, air-dried about 3 min and eluted in 70 µL of RNase-Free Water. For cultured samples the capacity is 1×10⁹ bacterial or fungal cells or fewer.
Specifications
| Format | Magnetic beads (Magbeads PN) with Lysis Tubes II |
|---|---|
| Sample types | Biological fluids including sputum, bronchoalveolar lavage fluid, blood and ascites |
| Sample input | 400 µL |
| Sample input (cultures) | ≤ 1×10⁹ bacterial or fungal cells |
| Nucleic acids recovered | DNA and RNA of viruses, bacteria and fungi |
| Elution volume | 70 µL RNase-Free Water |
Kit Components
| Component | 96 T |
|---|---|
| Buffer LBS-A | 20 mL |
| Buffer ML | 25 mL |
| Buffer GW1 | 180 mL |
| Buffer GW2 | 180 mL |
| RNase-Free Water | 10 mL |
| Proteinase K | 4 × 1.25 mL |
| Magbeads PN | 2 × 1.5 mL |
| Lysis Tubes II | 96 |
Applications
- Purification and enrichment of microbial DNA and RNA from biological fluids
- Nucleic acid extraction from sputum and bronchoalveolar lavage fluid
- Nucleic acid extraction from blood and ascites
- Template preparation for PCR, RT-PCR and qPCR
- Template preparation for NGS
Storage & Handling
All components are stored at room temperature (10–30°C) with a shelf life of 1 year.
Usage Notes
- Inspect the buffers for precipitate and dissolve them at 56°C if needed.
- Store samples at 2–8°C for the short term or at -20°C or -80°C for the long term.
- Avoid repeated freeze-thaw cycles of the sample.
- Maintain a clean work area and use DNA/RNA-free tips.
- Implement contamination controls throughout the procedure.
- Isopropanol is required at the binding step and is not supplied.
- Store the eluted nucleic acid at -20°C.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
