| Field | Specification |
|---|---|
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Product Overview
This kit provides a simple and efficient plant DNA extraction solution. DNA in the sample binds to the surface of silica-coated magnetic beads in the presence of high salt. After rinsing, the DNA is eluted in Buffer TB or deionized water. The extraction rate is closely related to the sample type and the cell disruption effect. The extracted DNA can be used for next-generation sequencing, PCR test and other downstream experiments.
The protocol processes 50–100 mg of fresh material or about 30 mg of dry material. The sample is lysed in 400 µL of Buffer PLS with 5 µL of RNase A at 70°C and 1600 rpm for 10 min, clarified at 12,000 rpm for 4 min, then bound with 550 µL of Buffer ZB and 20 µL of Magbeads for 5 min. The beads are washed twice with 650 µL of Buffer GW1 and twice with 650 µL of 75% ethanol, air-dried for 5–10 minutes and eluted in 100 µL of Buffer TB at 65°C for 10 min.
Specifications
| Format | Silica-coated magnetic beads (Magbeads PN) |
|---|---|
| Sample type | Plant material |
| Sample input | 50–100 mg fresh material or about 30 mg dry material |
| Binding chemistry | Silica-coated magnetic beads, high-salt binding |
| Special treatment | RNase A (10 mg/mL) supplied |
| Elution volume | 100 µL Buffer TB at 65°C for 10 min |
Kit Components
| Component | 96 T |
|---|---|
| Buffer PLS | 50 mL |
| Buffer ZB | 55 mL |
| Buffer GW1 (concentrate) | 80 mL |
| Buffer TB | 10 mL |
| RNase A (10 mg/mL) | 0.6 mL |
| Magbeads PN | 2 × 1 mL |
Applications
- DNA extraction from fresh or dry plant material
- Template preparation for next-generation sequencing
- Template preparation for PCR testing
Storage & Handling
All components are stored at room temperature (15–30°C) with a shelf life of 1 year.
Usage Notes
- Magbeads PN must not be frozen or subjected to high-speed centrifugation.
- For plants rich in polysaccharides and polyphenols, β-mercaptoethanol is added to 5%; it is not supplied with the kit. See the manufacturer's manual for the buffer to which it is added.
- Prepare the 75% ethanol wash separately, as it is not supplied.
- The extraction rate depends on the sample type and the cell disruption effect.
- Store the eluted DNA at -20°C.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
