| Field | Specification |
|---|---|
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Product Overview
This kit provides a simple and efficient automated plant RNA extraction solution. The RNA in the lysate binds to the surface of silica-coated magnetic beads in the presence of high salt, and is then washed with a rinse solution to remove impurities such as proteins. DNase I is then added to remove residual DNA, and the RNA is finally washed and eluted in RNase-Free Water. The total RNA obtained is of high purity, free of contamination from genomic DNA, proteins and other impurities, and can be used for Real Time RT-PCR, RT-PCR, Northern Blot, Dot Blot and in vitro translation and other downstream experiments.
The protocol processes 30–100 mg of fresh or frozen plant tissue. Tissue is ground to a powder under liquid nitrogen, lysed in 600 µL of Buffer PRL and clarified at 14,000 rpm and 4°C for 5 min. RNA is bound with 350 µL of Buffer PA and 20 µL of Magbeads PN at 1700 rpm for 5 min, washed with 500 µL of Buffer PGW1, treated with 80 µL of DNase I working solution for 5 min, washed twice with 700 µL of Buffer RW2, air-dried for 5–10 min and eluted in 100 µL of RNase-Free Water at 65°C and 1700 rpm for 10 min.
Specifications
| Format | Silica-coated magnetic beads (Magbeads PN) |
|---|---|
| Workflow | Suitable for automated extraction |
| Sample type | Fresh or frozen plant tissue |
| Sample input | 30–100 mg |
| Special treatment | DNase I (1 U/µL) with 10× Reaction Buffer supplied |
| Elution volume | 100 µL RNase-Free Water at 65°C, 1700 rpm, 10 min |
Kit Components
| Component | 96 T |
|---|---|
| DNase I (1 U/µL) | 2 × 1 mL |
| 10× Reaction Buffer | 1 mL |
| Buffer PRL | 60 mL |
| Buffer PA | 40 mL |
| Buffer PGW1 (concentrate) | 25 mL |
| Buffer RW2 (concentrate) | 40 mL |
| RNase-Free Water | 10 mL |
| Magbeads PN | 2 × 1 mL |
Applications
- Automated total RNA extraction from plant tissue
- Real Time RT-PCR and RT-PCR
- Northern Blot and Dot Blot analysis
- In vitro translation
Storage & Handling
DNase I and the 10× Reaction Buffer are stored at -20°C; the other components are stored at room temperature (15–30°C). The shelf life is 1 year at room temperature.
Usage Notes
- Use RNase-free materials and disposable gloves.
- Avoid high-starch plant tissues, which produce gelatinous substances.
- Use fresh or liquid nitrogen-frozen samples and avoid repeated freeze-thaw cycles.
- Prepare the DNase I mix fresh from 52 µL RNase-Free Water, 8 µL of buffer and 20 µL of DNase I.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
