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The Mahlavu cell line is a human hepatocellular carcinoma (HCC) cell line derived from an adult patient with liver cancer. Hepatocellular carcinoma is the most common type of primary liver cancer, often associated with chronic liver disease, including hepatitis B or C infection and cirrhosis. Mahlavu cells exhibit characteristics typical of aggressive liver cancer, such as high proliferative capacity, invasive behavior, and resistance to apoptosis, making them a valuable model for studying the molecular mechanisms underlying HCC progression and for testing potential anti-cancer therapies.
Mahlavu cells are known for their epithelial morphology and are typically cultured in conditions that support the growth of hepatic cells. These cells possess mutations in key oncogenes and tumor suppressor genes, which contribute to their tumorigenic properties. Researchers often use Mahlavu cells to study signaling pathways involved in HCC, such as the Wnt/β-catenin pathway, which is frequently dysregulated in liver cancers. Additionally, this cell line is useful in drug resistance studies, as it can provide insights into the mechanisms by which HCC cells evade standard chemotherapy treatments.
Due to its aggressive nature, the Mahlavu cell line is also employed in metastasis research. Studies involving these cells can help elucidate the processes by which liver cancer spreads to other organs, particularly the lungs and lymph nodes.
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- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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