Maleimide Plates

Maleimide Plates are ideal for assays that require site-directed orientation of specific biomolecules. The surface of these plates provides an effective platform for binding biomolecules with free sulfhydryl groups, such as peptides containing a terminal cysteine or thiol-containing haptens, as well as reducible disulfide bonds. These plates address challenges associated with coating these biomolecules onto standard polystyrene surfaces, offering enhanced binding and assay performance.

Maleimide Coated 96 Well Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Key Features

• Maleimide surface: Effective platform for binding biomolecules with free sulfhydryl groups.
• Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
• Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
• Wells design: flat bottom.
• Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
• Colors: Available in clear, white, and black. Customizable upper rim colors upon request. Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays. Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
  • Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
  • Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
• Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
• Alphanumeric Coding: For easy and accurate well identification during experiments.
• Recommended working volume: of 50 to 100 μl
• Packaging: Each coated plate is packed in a single barrier bag with desiccant
• Unit: Contains 5 plates
• Minimum order: 10 plates.
• Ready to use

Key Benefits

• Plates Design: The mould design provides optical quality, important to reduce the background signal. The rim protects the external face of the bottom from scratches
  • The mould design provides optical quality, important to reduce the background signal.
  • The rim protects the external face of the bottom from scratches
• Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
• Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
• Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
• Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Applications

• ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
• Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
• Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
• Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.