| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dual specificity mitogen-activated protein kinase kinase 6 (MAP kinase kinase 6, MAPKK 6)|MAPK/ERK kinase 6 (MEK 6)|Stress-activated protein kinase kinase 3 (SAPK kinase 3, SAPKK-3, SAPKK3)|MAP2K6|MEK6|MKK6|PRKMK6|SKK3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | mitogen-activated protein kinase kinase 6 |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes MAP2K6. It is validated for ELISA, Western Blot, Immunohistochemistry, Immunofluorescence.
Validated Applications
| Applications | ELISA, Western Blot, Immunohistochemistry, Immunofluorescence |
|---|---|
| WB | 1:500 - 1:2000 |
| IHC | 1:50 - 1:500 |
| IF | 1:50 - 1:100 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
mitogen-activated protein kinase kinase 6
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Immunogen | mitogen-activated protein kinase kinase 6 |
| Purification | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 37 kDa |
| UniProt # | P52564 |
| Gene ID | 5608 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Alternative Names | Dual specificity mitogen-activated protein kinase kinase 6 (MAP kinase kinase 6, MAPKK 6)|MAPK/ERK kinase 6 (MEK 6)|Stress-activated protein kinase kinase 3 (SAPK kinase 3, SAPKK-3, SAPKK3)|MAP2K6|MEK6|MKK6|PRKMK6|SKK3 |
Reactivity Notes
Validated reactivity: Human, Mouse. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
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Explore related antibodies and reagents for MAP2K6 research from Fine Test, or contact us for alternative formats.
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