| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human p38 alpha/MAPK14 was used as the immunogen for the MAPK14 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MAPK14 Antibody / Mitogen-activated protein kinase 14 / p38 alpha is a anti-MAPK14 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Flow cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: MAPK14
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): FACS, IP, IHC, WB
Biological background
MAPK14 is activated by upstream kinases MKK3 and MKK6 through dual phosphorylation of the conserved threonine-glycine-tyrosine (TGY) motif in its activation loop. Once activated, p38 alpha phosphorylates a wide array of targets including transcription factors (ATF2, MEF2C, ELK1), kinases (MAPKAPK2/3), and cell-cycle regulators, influencing inflammatory cytokine production, cell differentiation, and immune activation. It also regulates mRNA stability through the phosphorylation of RNA-binding proteins. In immune cells, p38 alpha drives the production of interleukin-6 and tumor necrosis factor-alpha, linking it to inflammatory diseases and autoimmune pathogenesis.
The MAPK14 antibody is widely used in molecular biology, inflammation, and cancer research to assess kinase expression, activation, and signal transduction. Western blot analysis typically identifies a 43 kilodalton band corresponding to MAPK14, while immunofluorescence and immunohistochemistry reveal cytoplasmic and nuclear staining depending on activation state. This antibody allows for monitoring of stress kinase activation and signaling pathway dynamics in diverse cell types.
MAPK14 is also implicated in tumor biology, as it influences cell migration, angiogenesis, and resistance to chemotherapy. Its inhibition has therapeutic potential in cancer, arthritis, and neuroinflammation. The MAPK14 antibody provides a reliable tool for studying stress-activated kinase pathways, cytokine regulation, and drug mechanism of action.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
