| Field | Specification |
|---|---|
| Applications | |
| Molecular weight | |
| Molecular formula | C34H30N4O3 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
MARK4 inhibitor 2 is an inhibitor of microtubule affinity-regulating kinase 4 (MARK4), with a Km of 6.3 × 10⁷ and an IC50 of 0.82 μM. It inhibits the growth of human cells and can be used for cancer research[1]. It is supplied as a light yellow to yellow solid (C34H30N4O3, MW 542.63) at 99.60% purity.
Physical & Chemical Properties
| Molecular Formula | C34H30N4O3 |
|---|---|
| Molecular Weight | 542.63 g/mol |
| Purity | 99.60% |
| Appearance | Solid |
| Color | Light yellow to yellow |
| SMILES | O=C(NC1=CC=C(C)C=C1)[C@@H](NC(CN(C2=C3C=CC=C2)C4=CC=C(C)C=C4C3=O)=O)CC5=CNC6=C5C=CC=C6 |
| Signaling Pathway | Cell Cycle/DNA Damage; Epigenetics; PI3K/Akt/mTOR |
| Solubility | In Vitro: DMSO: 100 mg/mL (184.29 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target
IC50: 0.82 μM (MARK4)[1] Km:6.3×10 7[1]
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
[1]. Maria Voura, et.al. Synthesis, Structural Modification, and Bioactivity Evaluation of Substituted Acridones as Potent Microtubule Affinity-Regulating Kinase 4 InhibitorsACS Pharmacology & Translational Science 2023 6 (7), 1052-1074.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 100 mg/mL (184.29 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | 2.5 mg/mL (4.61 mM); clear solution; requires sonication |
| How to prepare | Gives a clear solution at 2.5 mg/mL. Use with caution if continuous dosing will exceed two weeks. For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 900 μL corn oil. |
Data provided by the manufacturer.
In Vitro
In HeLa, U87MG, MDA-MB-435, U251 and HGFs cells, MARK4 inhibitor 2 (0-20 μM; 24 hour) suppresses growth, with EC50 values from 1.76 μM to 7.98 μM[1].
Cell Viability Assay[1]
| Cell Line | HeLa, U87MG, MDA-MB-435, U251, HGFs cells |
|---|---|
| Concentration | 0-20 μM |
| Incubation Time | 24 hours |
| Result | Inhibited the growth of human cells with EC50 s of 2.13, 4.13, 1.76, 7.98, 3.55 μM for HeLa, U87MG, MDA-MB-435, U251, HGFs cells, respectively. Decreased the proliferation of human cancer cells in the low micromolar range; also affects the non-cancerous HGF cells. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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