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The MC38 cell line is a murine model extensively utilized in colorectal carcinoma research. Originating from a colon adenocarcinoma in a C57BL/6 mouse, these cells exhibit a high mutational rate, particularly in the mutanome and neoantigen expression, making them highly sensitive to immune checkpoint inhibitor therapy. Their responsiveness to endogenous CD8+ T cell attacks against neoantigens underscores their value in studying immune interactions within tumor environments, positioning the MC38 model as a pivotal immunoresponsive murine tumor model.
MC38 cells form tumors and metastases in syngeneic C57BL6 murine hosts or immunocompromised mice. The MC38 colon adenocarcinoma model, especially when used in orthotopic mouse models, is recognized for its immunological responsiveness, rendering it an effective platform for evaluating immunotherapies, including radiation, checkpoint inhibitors, and other novel treatments.
MC38 cells express colon markers such as claudin-1 and SATB2, critical for investigating the genomic and epigenomic underpinnings of colorectal adenocarcinoma and for identifying potential treatments. The immunological characteristics of the MC38 xenograft model make it a versatile tool for cancer research, especially in the context of colorectal adenocarcinoma. The MC38 colon carcinoma model, with its high mutanome and neoantigen load, serves as an exemplary immunoresponsive murine model, facilitating the exploration of the complex dynamics between colorectal tumor cell lines and the host's immune system.
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- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS, 10 mM HEPES, NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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