| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C25H17Cl3FN3O3 |
| SMILES | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
MDM2/4-p53-IN-2 (2q) is a potent dual inhibitor of MDM2 and MDM4 that also activates p53, with IC50 values of 70.7 nM for the MDM2-p53 complex and 81.4 nM for the MDM4-p53 complex. It regulates the cell cycle, induces cell apoptosis, and has anticancer activity[1]. Its molecular formula is C25H17Cl3FN3O3, with a molecular weight of 532.78 g/mol.
Physical & Chemical Properties
| CAS Number | 3037300-49-2 |
|---|---|
| Molecular Formula | C25H17Cl3FN3O3 |
| Molecular Weight | 532.78 g/mol |
| SMILES | O=C(OCC)C([C@@H]1C2=CC=CC(Cl)=C2F)=NN([C@]13C(NC4=C3C=CC(Cl)=C4)=O)C5=CC=C(C=C5)Cl |
| Signaling Pathway | Apoptosis |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with your institution's chemical hygiene plan.
In Vitro
MDM2/4-p53-IN-2 (2q) (1-100 μM, 96 h) can inhibit cancer cell proliferation and induce apoptosis by targeting the G0/G1 cell cycle in a dose-dependent manner[1].
Cell Proliferation Assay[1]
| Cell Line | Human cancer cell lines HCT116, SJSA-1, MCF-7, LNCaP, human embryonic kidney epithelial cell line HEK 293T |
|---|---|
| Concentration | 1-100 μM |
| Incubation Time | 48 hours |
| Result | Showed anti-proliferative activity of HCT116 with an IC50 value of 18.0 μM. Showed well inhibition of cell viability against SJSA-1, MCF-7, LNCaP cells, all below 40%. Reduced cell viability of HEK 293T cells by 24%. |
Apoptosis Analysis[1]
| Cell Line | SJSA-1 cell lines |
|---|---|
| Concentration | 15 and 22.5 μM |
| Incubation Time | 96 hours |
| Result | Increased LDH release by 1.3-fold and 1.6-fold at concentrations of 15 μM and 22.5 μM, respectively. Caused a decrease in SJSA-1 cells, a significant decrease in cell growth and an increase in the number of early and late stage apoptotic cells at 22.5 μM. Induced a significant accumulation of G0/G1 phase cells with a percentage of 67.5% at 15 μM and 82.2% at 22.5 μM, while reducing the percentage of S and G2/M phase cells. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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