| Field | Specification |
|---|---|
| Mfr No | |
| Form | 2X Mastermix |
| Product Type | |
| Storage |
MegaFi™ Pro Fidelity 2X PCR MasterMix sets a new benchmark for specific, sensitive, robust, and high-fidelity PCR performance in a uniquely-formaulated buffer with gel loading dye. This advanced, strategically engineered enzyme is combined with a monoclonal antibody that inhibits both 5’-3’ polymerase activity and 3'-5’ exonuclease activity at room temperature, enabling precise Hot Start reactions. With exceptional sensitivity, MegaFi™ Pro can amplify even the most challenging templates, outperforming leading competitors. It delivers unparalleled high fidelity with an ultra-low error rate—over 2,000 times lower than Taq polymerase—making it the most accurate enzyme available. Ideal for demanding applications such as Next Generation Sequencing, molecular cloning, and diagnostics, MegaFi™ Pro ensures the highest quality results for your PCR needs.
Product Features:
- 2000X lower error rates than regular Taq
- Amplifies difficult templates
- Blunt ends
- Contains gel loading dye
- Included in abm's PCR Buffet Program
 |
ISO 13485:2016 MDSAP Certified |
Please refer to the product page for component information.
| Specification | Value |
|---|---|
| Enzyme Type | PCR Mastermix |
| Format | 2X Mastermix |
| Hot Start | No |
| Fidelity | High-Fidelity |
| 3'→5' Proofreading | Yes |
| Storage Conditions | Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
MegaFi Pro has high fidelity and high processivity. This means it has low error rates leading to more accurate sequences and longer amplicons leading to a faster reaction time. High fidelity is achieved due to its strong proofreading exonuclease activity, making it the preferred polymerase for all PCR applications requiring greater accuracy or the amplification of difficult templates.
Blunt ends, this makes it not suitable for TA cloning
It is the proofreading activity which digests nucleotides with 3´ hydroxyl groups from the 3´ to 5´ direction. This includes correction of mismatched base pairs and terminal 3´ digestion.
The loading dye does not interfere with downstream cloning experiments. However, PCR clean-up is still advised prior to cloning due to the presence of excess primers, salts, and other buffer components that could potentially affect cloning efficiency.
The fidelity of MegaFi Pro has been tested through Blue-white screening assay. LacZa gene was amplified using MegaFi™ Pro, NEB's Q5, Vazyme's PhantaMax, and Native Taq. The PCR amplicons were then subcloned into pUC19 vector and plated into a X-gal containing agar plate. Any mutations from PCR will cause the colonies to turn while no mutation will remain blue. Blue and white colonies were counted and the error rate per base was calculated and compared to native Taq.
It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
This product is abm’s next generation of DNA Polymerases and is functionally equivalent to Cat. G124, G124-dye, G464, G464-dye, G497, G497-dye, G886, and G897 (Precision, Bestaq, Kodaq, and MegaFi PCR MasterMixes), with improved performance. Contact our customer service team [contact ABM technical support] for more information.
The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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